Results 311 to 320 of about 3,664,932 (344)
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Bacterial Transformation (Kushner Method)
2003The principle of this procedure is very similar to that of the standard calcium chloride method, (see Chapter 34 ) i.e., exponential phase cells are harvested and washed with a solution containing divalent cations. This renders the cells competent, which simply means they are now able to take up DNA from the solution.
J W, Dale, P J, Greenaway
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2003
If one consults any genetics textbook written before the mid-1970s, one will find much on the transformation of such bacterial species as Bacillus subtilis, but no mention of Escherichia coli. The method now most generally used for introducing DNA into E.
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If one consults any genetics textbook written before the mid-1970s, one will find much on the transformation of such bacterial species as Bacillus subtilis, but no mention of Escherichia coli. The method now most generally used for introducing DNA into E.
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, 2020
Many studies demonstrated that CeO2 nanoparticles (NPs) could protect plant from stress and improve plant growth, with a great application potential in agriculture.
Yanhui Dai +7 more
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Many studies demonstrated that CeO2 nanoparticles (NPs) could protect plant from stress and improve plant growth, with a great application potential in agriculture.
Yanhui Dai +7 more
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"Steady-state" competence for bacterial transformation
Canadian Journal of Microbiology, 1968The cells of Bacillus subtilis, grown in minimal media, are known to become competent for transformation for a short period at a specific phase of ceil growth. In the present work the cells (strain 168 ind−) were grown in continuous culture (chemostat; glucose limiting, generation time 4 h, 37 °C).
H H, Eichhorn, S, Arikawa, S, Zamenhof
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Bacterial colony counting using distance transform
International Journal of Bio-Medical Computing, 1995A distance-transform based technique is presented for the segmentation of monochrome images of colonies grown on membrane filters. This is used to count the number of Escherichia coli in a given water sample, which is used as a parameter for determining water quality. The result is compared with fuzzy c-means clustering approach.
D P, Mukherjee +3 more
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Bacterial Transformations of Benzothiophene and Methylbenzothiophenes
Environmental Science & Technology, 1994The abilities of three Pseudomonas strains transform each of the six isomers of methylbenzothiophene was studied. These compounds would not support growth but were biotransformed in a cell suspension of one of the violates and in cultures of the other two isolates while they grew on 1-methylnaphthalene or glucose.
K G, Kropp +3 more
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Bacterial-induced transformation
Science Signaling, 2015Salmonella infection triggers signaling events that increase the probability of developing cancer in hosts that are genetically predisposed to transformation.
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Genomic Insights into Bacterial DMSP Transformations
Annual Review of Marine Science, 2012Genomic and functional genomic methods applied to both model organisms and natural communities have rapidly advanced understanding of bacterial dimethylsulfoniopropionate (DMSP) degradation in the ocean. The genes for the two main pathways in bacterial degradation, routing DMSP to distinctly different biogeochemical fates, have recently been identified.
Mary Ann, Moran +3 more
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Mechanism of Bacterial Transformation and Transfection
1974Publisher Summary This chapter discusses that in spite of the large amount of information on the physiology of the competent cell, including the production of competence factors in a number of transformation systems and an additional protein that must be synthesized to permit irreversible binding of DNA by D.
N K, Notani, J K, Setlow
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Bacterial transformation of pyrene in a marine environment
Oceanographic Literature Review, 1996The bacterial transformation of pyrene in an estuary environment, Kitimat Arm, British Columbia, Canada, was studied. A metabolite was separated from marine sediment and pore water samples and was identified as cis-4,5-dihydroxy-4,5-dihydropyrene, based ...
Xing-Fang Li +4 more
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