Results 1 to 10 of about 221,977 (219)

Optimizing Transformation Frequency of Cryptococcus neoformans and Cryptococcus gattii Using Agrobacterium tumefaciens

Journal of Fungi, 2021
The transformation of Cryptococcus spp. by Agrobacterium tumefaciens has proven to be a useful genetic tool. A number of factors affect transformation frequency. These factors include acetosyringone concentration, bacterial cell to yeast cell ratio, cell
Jianmin Fu, Nohelli E. Brockman, Brian L. Wickes   +2 more
doaj   +1 more source

Disinfectants facilitate the transformation of exogenous antibiotic resistance genes via multiple pathways

Ecotoxicology and Environmental Safety, 2023
The prevalence and spread of multidrug-resistant (MDR) bacteria pose a global challenge to public health. Natural transformation is one of the essential ways for horizontal transfer of antibiotic resistance genes (ARGs).
Yuqian Jia, Zeyu Wang, Shuyao Zhu, Zhiqiang Wang, Yuan Liu   +4 more
doaj   +1 more source

Optimization of the Transformation Protocol for Increased Efficiency of Genetic Transformation in Hevea brasiliensis

Plants, 2022
The recurring growth of bacterium in newly developed resistant cells and a minimal level of bacterial infection rate are the main limiting factors of Agrobacterium-mediated transformation experiments in Hevea brasiliensis.
Jinu Udayabhanu, Tiandai Huang, Shichao Xin, Jing Cheng, Yuwei Hua, Huasun Huang   +5 more
doaj   +1 more source

Impacts of deforestation and forest regeneration on soil bacterial communities associated with phosphorus transformation processes in the Brazilian Amazon region

Ecological Indicators, 2023
Land-use change has negative impacts on the biodiversity of plants and animals. However, we lack studies and/or information about the impacts of land-use change on the biodiversity of soil microorganisms, especially those involved in the phosphorus (P ...
Alexandre Pedrinho, Lucas William Mendes, Felipe Martins do Rêgo Barros, Luis Fernando Merloti, Mayara Martins e Martins, Simone Raposo Cotta, Fernando Dini Andreote, Siu Mui Tsai   +7 more
doaj   +1 more source

Bacterial Transformation Buffers Environmental Fluctuations through the Reversible Integration of Mobile Genetic Elements

mBio, 2020
Horizontal gene transfer (HGT) promotes the spread of genes within bacterial communities. Among the HGT mechanisms, natural transformation stands out as being encoded by the bacterial core genome.
Gabriel Carvalho, David Fouchet, Gonché Danesh, Anne-Sophie Godeux, Maria-Halima Laaberki, Dominique Pontier, Xavier Charpentier, Samuel Venner   +7 more
doaj   +1 more source

Integrated FT-ICR MS and metabolome reveals diatom-derived organic matter by bacterial transformation under warming and acidification

iScience, 2023
Summary: Bacterial transformation and processing of diatom-derived organic matter (OM) is extremely important for the cycling of production and energy in marine ecosystems; this process contributes to the production of microbial food webs. In this study,
Yang Liu, Chao Ma, Jun Sun
doaj   +1 more source

Mechanisms of Transforming DNA Uptake to the Periplasm of Bacillus subtilis

mBio, 2021
Transformation is a widely distributed mechanism of bacterial horizontal gene transfer that plays a role in the spread of antibiotic resistance and virulence genes and more generally in evolution.
Jeanette Hahn, Micaela DeSantis, David Dubnau   +2 more
doaj   +1 more source

A novel method for DNA delivery into bacteria using cationic copolymers [PDF]

Brazilian Journal of Medical and Biological Research, 2021
Amphiphilic copolymers have a wide variety of medical and biotechnological applications, including DNA transfection in eukaryotic cells. Still, no polymer-primed transfection of prokaryotic cells has been described.
V.V. de Souza, P.A.M. Vitale, F.H. Florenzano, R.K. Salinas, I.M. Cuccovia   +4 more
doaj   +1 more source

Altering under-represented DNA sequences elevates bacterial transformation efficiency

mBio, 2023
A cornerstone of bacterial molecular biology is the ability to genetically manipulate the microbe under study. Many bacteria are difficult to manipulate genetically, a phenotype due in part to robust removal of newly acquired DNA, for example, by ...
Shuai Hu, Stefani Giacopazzi, Ryan Modlin, Kevin Karplus, David L. Bernick, Karen M. Ottemann   +5 more
doaj   +1 more source

Disclosing the ecological implications of heavy metal disturbance on the microbial N-transformation process in the ocean tidal flushing urban estuary

Ecological Indicators, 2022
Anthropogenic source of heavy metals in the estuary-coastal sediment could cause serious environmental concerns. Thus, much needs to be concerned about the linkages between bacterial community, microbial nitrogen processes and heavy metals accumulation ...
Caixia Wang, Jiujun Ju, Haikun Zhang, Pengyuan Liu, Qiusheng Zheng, Xiaoke Hu   +5 more
doaj   +1 more source