Results 21 to 30 of about 2,649,246 (260)

Citric acid/β-alanine carbon dots as a novel tool for delivery of plasmid DNA into E. coli cells

Scientific Reports, 2021
Successful delivery of plasmid DNA into the microbial cells is fundamental in recombinant DNA technology. Natural bacterial transformation is limited to only certain species due in part to the repulsive forces between negatively charged DNA and bacterial
Anju Pandey, Asmita Devkota, Anil Sigdel, Zeinab Yadegari, Korsi Dumenyo, Ali Taheri   +5 more
doaj   +1 more source

Obtaining of transgenic potato (Solanum tuberosum L.) cultivar IPB CP3 containing LYZ‐C gene resistant to bacterial wilt disease

Indonesian Journal of Biotechnology, 2021
Bacterial wilt caused by Ralstonia solanacearum is one of the most important bacterial diseases in potato production. This study aimed to obtain the transgenic potato (Solanum tuberosum L.) cultivar IPB CP3, containing LYZ‐C gene encoding for lysozyme ...
Pasmawati Pasmawati, Aris Tjahjoleksono, Suharsono Suharsono   +2 more
doaj   +1 more source

Le mécanisme de la fermentation malolactique des vins

OENO One, 1977
Dans ce travail on étudie l'activité de suspensions bactériennes responsables de la fermentation malolactique des vins, on décrit les méthodes de purification d'extraits bactériens catalysant cette transformation.
M. Lonvaud, Aline Lonvaud-Funel, Pascal Ribéreau-Gayon   +2 more
doaj   +1 more source

Bacterial Lipopolysaccharide Augmented Malignant Transformation and Promoted the Stemness in Prostate Cancer Epithelial Cells

Journal of Inflammation Research, 2021
Sijie Tang,1,2 Xueqi Lian,1 Huiying Cheng,1 Jiaqian Guo,1 Daguang Ni,1 Can Huang,1 Xiang Gu,2 Hong Meng,3 Jiajia Jiang,1 Xiaohua Li1,4,5 1The Aoyang Cancer Institute, Affiliated Aoyang Hospital of Jiangsu University, Zhangjiagang, Suzhou, 215600, People ...
Tang S, Lian X, Cheng H, Guo J, Ni D, Huang C, Gu X, Meng H, Jiang J, Li X   +9 more
doaj  

A Fast and Practical Yeast Transformation Method Mediated by Escherichia coli Based on a Trans-Kingdom Conjugal Transfer System: Just Mix Two Cultures and Wait One Hour. [PDF]

PLoS ONE, 2016
Trans-kingdom conjugation is a phenomenon by which DNA is transferred into a eukaryotic cell by a bacterial conjugal transfer system. Improvement in this method to facilitate the rapid co-cultivation of donor bacterial and recipient eukaryotic cell ...
Kazuki Moriguchi, Shinji Yamamoto, Yuta Ohmine, Katsunori Suzuki   +3 more
doaj   +1 more source

Stable nuclear transformation of Gonium pectorale [PDF]

, 2009
Lerche K, Hallmann A. Stable nuclear transformation of Gonium pectorale. BMC Biotechnology. 2009;9(1): 64.BACKGROUND: Green algae of the family Volvocaceae are a model lineage for studying the molecular evolution of multicellularity and cellular ...
Lerche, Kai, Hallmann Armin, Hallmann, Armin ; https://orcid.org/, Lerche Kai, Armin Hallmann, Kai Lerche   +5 more
core   +1 more source

Markerless gene deletion in Ralstonia solanacearum based on its natural transformation competence

Frontiers in Microbiology, 2022
Ralstonia solanacearum species complex (RSSC) is a group of Gram-negative bacterial pathogen capable of infecting numerous plants and crops, causing severe vascular wilt diseases.
Jinli Yan, Jinli Yan, Nuoqiao Lin, Nuoqiao Lin, Xiaoqing Wang, Xiaoqing Wang, Xuemei Chen, Xuemei Chen, Huishan Wang, Huishan Wang, Qiqi Lin, Qiqi Lin, Xiaofan Zhou, Lianhui Zhang, Lianhui Zhang, Lisheng Liao   +15 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Metagenomic Insights into How Understory Vegetation Enhances Soil Nitrogen Availability via Microbial Nitrogen Transformation in Poplar Plantations

Agronomy
Long-term monoculture of poplar plantations for industrial material production has been widely reported to cause severe soil degradation, while the presence of understory vegetation might enhance soil nitrogen (N) transformation and supply.
Wenyu Jia, Tong Li, Peilei Ye, Yuxin Chen, Ruoning Zhu, Ruixin Yan, Haoran Yue, Ye Tian   +7 more
doaj   +1 more source

Mechanism of transformation in Mycobacteria using a novel shockwave assisted technique driven by in-situ generated oxyhydrogen

Scientific Reports, 2017
We present a novel method for shockwave-assisted bacterial transformation using a miniature oxyhydrogen detonation-driven shock tube. We have obtained transformation efficiencies of about 1.28 × 106, 1.7 × 106, 5 × 106, 1 × 105, 1 × 105 and 2 × 105 ...
Akshay Datey, Janardhanraj Subburaj, Jagadeesh Gopalan, Dipshikha Chakravortty   +3 more
doaj   +1 more source