Results 21 to 30 of about 221,977 (219)

Bacterial Lipopolysaccharide Augmented Malignant Transformation and Promoted the Stemness in Prostate Cancer Epithelial Cells

Journal of Inflammation Research, 2021
Sijie Tang,1,2 Xueqi Lian,1 Huiying Cheng,1 Jiaqian Guo,1 Daguang Ni,1 Can Huang,1 Xiang Gu,2 Hong Meng,3 Jiajia Jiang,1 Xiaohua Li1,4,5 1The Aoyang Cancer Institute, Affiliated Aoyang Hospital of Jiangsu University, Zhangjiagang, Suzhou, 215600, People ...
Tang S, Lian X, Cheng H, Guo J, Ni D, Huang C, Gu X, Meng H, Jiang J, Li X   +9 more
doaj  

Markerless gene deletion in Ralstonia solanacearum based on its natural transformation competence

Frontiers in Microbiology, 2022
Ralstonia solanacearum species complex (RSSC) is a group of Gram-negative bacterial pathogen capable of infecting numerous plants and crops, causing severe vascular wilt diseases.
Jinli Yan, Jinli Yan, Nuoqiao Lin, Nuoqiao Lin, Xiaoqing Wang, Xiaoqing Wang, Xuemei Chen, Xuemei Chen, Huishan Wang, Huishan Wang, Qiqi Lin, Qiqi Lin, Xiaofan Zhou, Lianhui Zhang, Lianhui Zhang, Lisheng Liao   +15 more
doaj   +1 more source

A Fast and Practical Yeast Transformation Method Mediated by Escherichia coli Based on a Trans-Kingdom Conjugal Transfer System: Just Mix Two Cultures and Wait One Hour. [PDF]

PLoS ONE, 2016
Trans-kingdom conjugation is a phenomenon by which DNA is transferred into a eukaryotic cell by a bacterial conjugal transfer system. Improvement in this method to facilitate the rapid co-cultivation of donor bacterial and recipient eukaryotic cell ...
Kazuki Moriguchi, Shinji Yamamoto, Yuta Ohmine, Katsunori Suzuki   +3 more
doaj   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Metagenomic Insights into How Understory Vegetation Enhances Soil Nitrogen Availability via Microbial Nitrogen Transformation in Poplar Plantations

Agronomy
Long-term monoculture of poplar plantations for industrial material production has been widely reported to cause severe soil degradation, while the presence of understory vegetation might enhance soil nitrogen (N) transformation and supply.
Wenyu Jia, Tong Li, Peilei Ye, Yuxin Chen, Ruoning Zhu, Ruixin Yan, Haoran Yue, Ye Tian   +7 more
doaj   +1 more source

Mechanism of transformation in Mycobacteria using a novel shockwave assisted technique driven by in-situ generated oxyhydrogen

Scientific Reports, 2017
We present a novel method for shockwave-assisted bacterial transformation using a miniature oxyhydrogen detonation-driven shock tube. We have obtained transformation efficiencies of about 1.28 × 106, 1.7 × 106, 5 × 106, 1 × 105, 1 × 105 and 2 × 105 ...
Akshay Datey, Janardhanraj Subburaj, Jagadeesh Gopalan, Dipshikha Chakravortty   +3 more
doaj   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Bacterial bioprinting on a flexible substrate for fabrication of a colorimetric temperature indicator by using a commercial inkjet printer

Journal of Medical Signals and Sensors, 2018
Background: Bacterial sensors are recommended for medical sciences, pharmaceutical industries, food industries, and environmental monitoring due to low cost, high sensitivity, and appropriate response time.
Zeinab Mohammadi, Mohsen Rabbani
doaj   +1 more source

Structure-function studies reveal ComEA contains an oligomerization domain essential for transformation in gram-positive bacteria

Nature Communications, 2022
ComEA is a DNA-binding protein required for DNA uptake during bacterial transformation. Here, Ahmed et al. determine X-ray crystal structures of ComEA from Gram-positive bacteria, identifying a domain that is absent in Gram-negative bacteria and drives ...
Ishtiyaq Ahmed, Jeanette Hahn, Amy Henrickson, Faisal Tarique Khaja, Borries Demeler, David Dubnau, Matthew B. Neiditch   +6 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source