Results 11 to 20 of about 3,877,619 (352)
Altering under-represented DNA sequences elevates bacterial transformation efficiency [PDF]
mBio, 2023 A cornerstone of bacterial molecular biology is the ability to genetically manipulate the microbe under study. Many bacteria are difficult to manipulate genetically, a phenotype due in part to robust removal of newly acquired DNA, for example, by ...
Shuai Hu +5 more
doaj +3 more sources
Transformation Asymmetry and the Evolution of the Bacterial Accessory Genome [PDF]
Molecular Biology and Evolution, 2017 Bacterial transformation can insert or delete genomic islands (GIs), depending on the donor and recipient genotypes, if an homologous recombination spans the GI's integration site and includes sufficiently long flanking homologous arms. Combining mathematical models of recombination with experiments using pneumococci found GI insertion rates declined ...
Apagyi, K, Fraser, C, Croucher, N
openaire +7 more sources
Room temperature electrocompetent bacterial cells improve DNA transformation and recombineering efficiency [PDF]
Scientific Reports, 2016 Bacterial competent cells are essential for cloning, construction of DNA libraries, and mutagenesis in every molecular biology laboratory. Among various transformation methods, electroporation is found to own the best transformation efficiency.
Qiang Tu +13 more
semanticscholar +2 more sources
Steady at the wheel: conservative sex and the benefits of bacterial transformation [PDF]
bioRxiv, 2016 Many bacteria are highly sexual, but the reasons for their promiscuity remain obscure. Did bacterial sex evolve to maximize diversity and facilitate adaptation in a changing world, or does it instead help to retain the bacterial functions that work right
O. Ambur +4 more
semanticscholar +3 more sources
Coexisting protist-bacterial community accelerates protein transformation in microcosm experiments [PDF]
Frontiers in Marine Science, 2014 Proteins constitute the major portion of labile substances in the marine environment and are an important source of organic matter supporting marine ecosystems.
Ngo Vy Thao +4 more
doaj +2 more sources
Synchronized Electromechanical Shock Wave-Induced Bacterial Transformation [PDF]
ACS Omega, 2019 We report a simple device that generates synchronized mechanical and electrical pressure waves for carrying out bacterial transformation. The mechanical pressure waves are produced by igniting a confined nanoenergetic composite material that provides ...
Rishi Kant +11 more
doaj +2 more sources
Bacterial transformation: ComFA is a DNA‐dependent ATPase that forms complexes with ComFC and DprA [PDF]
Molecular Microbiology, 2017 International audiencePneumococcal natural transformation contributes to genomic plasticity, antibiotic resistance development and vaccine escape. Streptococcus pneumoniae, like many other naturally transformable species, has evolved sophisticated ...
Amy Diallo +9 more
semanticscholar +2 more sources
Ecological Indicators, 2023 Land-use change has negative impacts on the biodiversity of plants and animals. However, we lack studies and/or information about the impacts of land-use change on the biodiversity of soil microorganisms, especially those involved in the phosphorus (P ...
Alexandre Pedrinho +7 more
doaj +1 more source
Bacterial transformations of naphthothiophenes [PDF]
Applied and Environmental Microbiology, 1997 Vol. 63, no. 9, p. 3468, Table 1, subheading under heading "Total recovery": "((delta)mol)" should read "((mu)mol)." [This corrects the article on p. 3463 in vol. 63.].
K G, Kropp, J T, Andersson, P M, Fedorak
openaire +4 more sources
Alternative design of a bacterial transformation chip [PDF]
, 2022 Traditionally, bacterial transformations are carried in small microcentrifuge tubes. But, there is a possibility that spatial confinement in small microchannels can facilitate plasmid entry into competent cells.
Wenfa Ng (9593279)
core +1 more source