Homogenous M13 bacteriophage quantification assay using switchable lanthanide fluorescence probes
We have developed a rapid and reliable bacteriophage quantification method based on measurement of phage single-stranded DNA (ssDNA) using switchable lanthanide chelate complementation probes.
Ari Lehmusvuori +4 more
doaj +1 more source
Two different evolutionary lines of filamentous phages in Ralstonia solanacearum: their effects on bacterial virulence [PDF]
The integration and excision of various filamentous phage genomes into and out of their host chromosomes occurs by site-specific recombination. The mechanisms proposed for these events include reactions mediated by phage-encoded recombinases and host ...
Ahmed Askora, Takashi Yamada
core +2 more sources
Structural characterization of S. mansoni cathepsin D1 in complex with a nanobody reveals the conformation of the propeptide‐bound state and identifies a schistosome‐specific epitope.Schistosoma mansoni cathepsin D1 (SmCD1) has been shown to be an essential enzyme for helminth metabolism due to its role in haemoglobin degradation: a key amino‐acid ...
Kelly L. Parker +7 more
wiley +1 more source
Spectroscopic characterization of reaction centers of the (M)Y210W mutant of the photosynthetic bacterium Rhodobacter sphaeroides [PDF]
The tyrosine-(M)210 of the reaction center of Rhodobacter sphaeroides 2.4.1 has been changed to a tryptophan using site-directed mutagenesis. The reaction center of this mutant has been characterized by low-temperature absorption and fluorescence ...
Arlt, Thomas +10 more
core +1 more source
M13-templated magnetic nanoparticles for targeted in vivo imaging of prostate cancer [PDF]
Molecular imaging allows clinicians to visualize the progression of tumours and obtain relevant information for patient diagnosis and treatment1. Owing to their intrinsic optical, electrical and magnetic properties, nanoparticles are promising contrast ...
Belcher, Angela M +6 more
core +1 more source
Nanoscale bacteriophage biosensors beyond phage display
Jong-Wook Lee,1 Jangwon Song,1,2 Mintai P Hwang,1 Kwan Hyi Lee1,2 1Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, Korea; 2Department of Biomedical Engineering, University of Science and ...
Lee JW, Song J, Hwang MP, Lee KH
doaj
Polymerase chain reaction on a viral nanoparticle [PDF]
The field of synthetic biology includes studies that aim to develop new materials and devices from biomolecules. In recent years much work has been carried out using a range of biomolecular chassis including α-helical coiled coils, α-sheet amyloids and ...
Carr-Smith, James +11 more
core +1 more source
Detection and precipitation of hepatitis B core antigen using a fusion bacteriophage [PDF]
The nucleocapsids of hepatitis B virus (HBV) are made of 180 or 240 subunits of core proteins or known as core antigens (HBcAg). A fusion bacteriophage bearing the WSFFSNI sequence that interacts tightly to HBcAg was employed as a diagnostic reagent for ...
Hasmoni, Siti Salwa +2 more
core +1 more source
大腸菌を用いたフォスファカン(コンドロイチン硫酸プロテオグリカン)の融合コア蛋白の発現条件の検討 [PDF]
Optimal conditions for expressing a specific region of core protein of phosphacan, a chondroitin sulfate proteoglycan known as receptor type protein tyrosine phosphatase, as fusion protein with glutathione S-transferase (GST) in E.coli were examined. DNA
Ito, Sekiko, Okamoto, Motoi
core +1 more source
Engineered cell-cell communication via DNA messaging
Background Evolution has selected for organisms that benefit from genetically encoded cell-cell communication. Engineers have begun to repurpose elements of natural communication systems to realize programmed pattern formation and coordinate other ...
Ortiz Monica E, Endy Drew
doaj +1 more source

