Results 1 to 10 of about 29,478 (285)

Single molecule kinetics of bacteriorhodopsin by HS-AFM [PDF]

Nature Communications, 2021
Here, the authors use high-speed atomic force microscopy (HS-AFM) methods to characterize the single molecule kinetics of wild-type bacteriorhodopsin (bR) with millisecond temporal resolution, providing new insights into the bR conformational cycle.
Alma P. Perrino, Atsushi Miyagi, Simon Scheuring   +2 more
doaj   +3 more sources

Bacteriorhodopsin of purple membrane reverses anisotropy outside the pH range of proton pumping based on logic gate realization [PDF]

Scientific Reports
The bacteriorhodopsin of purple membrane is the first discovered light-sensing protein among ion transporting microbial rhodopsins, some of which (e.g.
Hamdy I. A. Mostafa, Abdo A. Elfiki
doaj   +2 more sources

Engineering of soluble bacteriorhodopsin. [PDF]

Chem Sci
Analogues of membrane protein bacteriorhodopsin are reported that are soluble and stable, bind retinal and exhibit photocycles under illumination, highlighting the power of modern protein engineering approaches.
Nikolaev A, Orlov Y, Tsybrov F, Kuznetsova E, Shishkin P, Kuzmin A, Mikhailov A, Nikolaeva YS, Anuchina A, Chizhov I, Semenov O, Kapranov I, Borshchevskiy V, Remeeva A, Gushchin I.   +14 more
europepmc   +3 more sources

Lipidic cubic phase serial millisecond crystallography using synchrotron radiation [PDF]

IUCrJ, 2015
Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins. Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron ...
Przemyslaw Nogly, Daniel James, Dingjie Wang, Thomas A. White, Nadia Zatsepin, Anastasya Shilova, Garrett Nelson, Haiguang Liu, Linda Johansson, Michael Heymann, Kathrin Jaeger, Markus Metz, Cecilia Wickstrand, Wenting Wu, Petra Båth, Peter Berntsen, Dominik Oberthuer, Valerie Panneels, Vadim Cherezov, Henry Chapman, Gebhard Schertler, Richard Neutze, John Spence, Isabel Moraes, Manfred Burghammer, Joerg Standfuss, Uwe Weierstall   +26 more
doaj   +7 more sources

Retinal photoisomerization versus counterion protonation in light and dark-adapted bacteriorhodopsin and its primary photoproduct [PDF]

Nature Communications
Discovered over 50 years ago, bacteriorhodopsin is the first recognized and most widely studied microbial retinal protein. Serving as a light-activated proton pump, it represents the archetypal ion-pumping system.
Partha Malakar, Samira Gholami, Mohammad Aarabi, Ivan Rivalta, Mordechai Sheves, Marco Garavelli, Sanford Ruhman   +6 more
doaj   +2 more sources

The Role of Graphene Monolayers in Enhancing the Yield of Bacteriorhodopsin Photostates for Optical Memory Applications [PDF]

Applied Sciences, 2021
Bacteriorhodopsin (bR) is a photoactive protein that has gained increasing importance as a tool for optical memory storage due to its remarkable photochemical and thermal stability.
Roma Patel, Gregory Salamone, Isaac Macwan   +2 more
doaj   +2 more sources

Reversible Photochromic Reactions of Bacteriorhodopsin from Halobacterium salinarum at Femto- and Picosecond Times [PDF]

Molecules
The operation of bacteriorhodopsin (BR) from the archaeon Halobacterium salinarum is based on the photochromic reaction of isomerization of the chromophore group (the retinal protonated Schiff base, RPSB) from the all-trans to the 13-cis form.
Olga Smitienko, Tatyana Feldman, Ivan Shelaev, Fedor Gostev, Arseniy Aybush, Dmitry Cherepanov, Victor Nadtochenko, Mikhail Ostrovsky   +7 more
doaj   +2 more sources

Structural effects of high laser power densities on an early bacteriorhodopsin photocycle intermediate [PDF]

Nature Communications
Time-resolved serial crystallography at X-ray Free Electron Lasers offers the opportunity to observe ultrafast photochemical reactions at the atomic level.
Quentin Bertrand, Przemyslaw Nogly, Eriko Nango, Demet Kekilli, Georgii Khusainov, Antonia Furrer, Daniel James, Florian Dworkowski, Petr Skopintsev, Sandra Mous, Isabelle Martiel, Per Börjesson, Giorgia Ortolani, Chia-Ying Huang, Michal Kepa, Dmitry Ozerov, Steffen Brünle, Valerie Panneels, Tomoyuki Tanaka, Rie Tanaka, Kensuke Tono, Shigeki Owada, Philip J. M. Johnson, Karol Nass, Gregor Knopp, Claudio Cirelli, Christopher Milne, Gebhard Schertler, So Iwata, Richard Neutze, Tobias Weinert, Jörg Standfuss   +31 more
doaj   +2 more sources

The Effect of Bacterioruberin Deletion on Production of Bacteriorhodopsin in Halobacterium salinarum R1 [PDF]

Journal of Pure and Applied Chemistry Research, 2018
Bacteriorhodopsin is a retinal protein located in purple membrane of Halobacterium salinarum which acts as light-dependent proton pump. Bacterioruberin is a by-product in bacteriorhodopsin biosynthesis pathway in Halobacterium salinarum.
Abbas Karimi Fard, Ali Asghar Deldar, Saeed Sedaghat   +2 more
doaj   +1 more source

Protein Motifs for Proton Transfers That Build the Transmembrane Proton Gradient

Frontiers in Chemistry, 2021
Biological membranes are barriers to polar molecules, so membrane embedded proteins control the transfers between cellular compartments. Protein controlled transport moves substrates and activates cellular signaling cascades.
Divya Kaur, Divya Kaur, Umesh Khaniya, Umesh Khaniya, Yingying Zhang, Yingying Zhang, M. R. Gunner, M. R. Gunner, M. R. Gunner   +8 more
doaj   +1 more source