Results 1 to 10 of about 17,342 (214)

Bacteriorhodopsin of purple membrane reverses anisotropy outside the pH range of proton pumping based on logic gate realization [PDF]

Scientific Reports
The bacteriorhodopsin of purple membrane is the first discovered light-sensing protein among ion transporting microbial rhodopsins, some of which (e.g.
Hamdy I. A. Mostafa, Abdo A. Elfiki
doaj   +2 more sources

Engineering of soluble bacteriorhodopsin. [PDF]

Chem Sci
Analogues of membrane protein bacteriorhodopsin are reported that are soluble and stable, bind retinal and exhibit photocycles under illumination, highlighting the power of modern protein engineering approaches.
Nikolaev A, Orlov Y, Tsybrov F, Kuznetsova E, Shishkin P, Kuzmin A, Mikhailov A, Nikolaeva YS, Anuchina A, Chizhov I, Semenov O, Kapranov I, Borshchevskiy V, Remeeva A, Gushchin I.   +14 more
europepmc   +3 more sources

Retinal photoisomerization versus counterion protonation in light and dark-adapted bacteriorhodopsin and its primary photoproduct [PDF]

Nature Communications
Discovered over 50 years ago, bacteriorhodopsin is the first recognized and most widely studied microbial retinal protein. Serving as a light-activated proton pump, it represents the archetypal ion-pumping system.
Partha Malakar, Samira Gholami, Mohammad Aarabi, Ivan Rivalta, Mordechai Sheves, Marco Garavelli, Sanford Ruhman   +6 more
doaj   +2 more sources

Lipidic cubic phase serial millisecond crystallography using synchrotron radiation [PDF]

IUCrJ, 2015
Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins. Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron ...
Przemyslaw Nogly, Daniel James, Dingjie Wang, Thomas A. White, Nadia Zatsepin, Anastasya Shilova, Garrett Nelson, Haiguang Liu, Linda Johansson, Michael Heymann, Kathrin Jaeger, Markus Metz, Cecilia Wickstrand, Wenting Wu, Petra Båth, Peter Berntsen, Dominik Oberthuer, Valerie Panneels, Vadim Cherezov, Henry Chapman, Gebhard Schertler, Richard Neutze, John Spence, Isabel Moraes, Manfred Burghammer, Joerg Standfuss, Uwe Weierstall   +26 more
doaj   +7 more sources

Reversible Photochromic Reactions of Bacteriorhodopsin from Halobacterium salinarum at Femto- and Picosecond Times [PDF]

Molecules
The operation of bacteriorhodopsin (BR) from the archaeon Halobacterium salinarum is based on the photochromic reaction of isomerization of the chromophore group (the retinal protonated Schiff base, RPSB) from the all-trans to the 13-cis form.
Olga Smitienko, Tatyana Feldman, Ivan Shelaev, Fedor Gostev, Arseniy Aybush, Dmitry Cherepanov, Victor Nadtochenko, Mikhail Ostrovsky   +7 more
doaj   +2 more sources

The Effect of Bacterioruberin Deletion on Production of Bacteriorhodopsin in Halobacterium salinarum R1 [PDF]

Journal of Pure and Applied Chemistry Research, 2018
Bacteriorhodopsin is a retinal protein located in purple membrane of Halobacterium salinarum which acts as light-dependent proton pump. Bacterioruberin is a by-product in bacteriorhodopsin biosynthesis pathway in Halobacterium salinarum.
Abbas Karimi Fard, Ali Asghar Deldar, Saeed Sedaghat   +2 more
doaj   +1 more source

Protein Motifs for Proton Transfers That Build the Transmembrane Proton Gradient

Frontiers in Chemistry, 2021
Biological membranes are barriers to polar molecules, so membrane embedded proteins control the transfers between cellular compartments. Protein controlled transport moves substrates and activates cellular signaling cascades.
Divya Kaur, Divya Kaur, Umesh Khaniya, Umesh Khaniya, Yingying Zhang, Yingying Zhang, M. R. Gunner, M. R. Gunner, M. R. Gunner   +8 more
doaj   +1 more source

Peptide-Coated Bacteriorhodopsin-Based Photoelectric Biosensor for Detecting Rheumatoid Arthritis

Biosensors, 2023
An effective early diagnosis is important for rheumatoid arthritis (RA) management. This study reveals a novel RA detection method using bacteriorhodopsin as a photoelectric transducer, a light-driven proton pump in purple membranes (PMs). It was devised
Hsiu-Mei Chen, Yi-Hsuan Tsai, Chien-Yi Hsu, Yong-Yi Wang, Cheng-En Hsieh, Jin-Hua Chen, Yu-Sheng Chang, Ching-Yu Lin   +7 more
doaj   +1 more source

Small-Molecule Analysis Based on DNA Strand Displacement Using a Bacteriorhodopsin Photoelectric Transducer: Taking ATP as an Example

Sensors, 2023
A uniformly oriented purple membrane (PM) monolayer containing photoactive bacteriorhodopsin has recently been applied as a sensitive photoelectric transducer to assay color proteins and microbes quantitatively.
Hsiu-Mei Chen, Wen-Chang Wang, Hong-Ren Chen   +2 more
doaj   +1 more source

Spontaneous and Electrically Induced Anisotropy of Composite Agarose Gels

Gels, 2022
Agarose gels containing and not bacteriorhodopsin purple membranes (incorporated before gelling) manifest spontaneous optical anisotropy. The dependencies of the anisotropy on the agarose concentration and time have been studied.
Alexandar M. Zhivkov, Svetlana H. Hristova   +1 more
doaj   +1 more source