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, 2007 The production of a recombinant baculovirus expression vector normally involves mixing infectious virus DNA with a plasmid-based transfer vector and then cotransfecting insect cells to initiate virus infection. The aim of this chapter is to provide an update on the range of baculovirus transfer vectors currently available.
Possee, Robert D., King, Linda A.
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Fundamentals of Baculovirus Expression and Applications
Advances in Experimental Medicine and Biology, 2016In 1982 E. coli produced human insulin, the world's first recombinant DNA drug, was approved by the FDA. Since this historical event, remarkable progress has been made in developing bacterial, yeast, mammalian and insect cell protein expression systems that are used to produce recombinant proteins for both research and clinical applications.
Thomas A, Kost, Christopher W, Kemp
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Journal of Biotechnology, 2013 Genetically transformed lepidopteran insect cell lines have biotechnological applications as constitutive recombinant protein production platforms and improved hosts for baculovirus-mediated recombinant protein production.
Chi-Hung Lin, Donald L Jarvis
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Microbiota modulates gut immunity and promotes baculovirus infection in Helicoverpa armigera
Insect Science, 2021Baculoviruses are natural enemies of agricultural and forest insect pests and play an important role in biological pest control. Oral infection by baculovirus in the insect midgut is necessary for establishing systemic infection and eventually killing ...
Chuanfei Yuan +4 more
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Manipulation of baculovirus vectors
Molecular Biotechnology, 1997Baculovirus expression vectors provide an excellent system for the synthesis of recombinant proteins in insect cells. This article presents sufficient background information to allow the nonspecialist to understand the basic principles of the technology and the development of baculovirus expression vectors.
C L, Merrington +2 more
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Baculovirus Phylogeny and Evolution
Current Drug Targets, 2007The family Baculoviridae represents one of the largest and most diverse groups of viruses and a unique model for studying the forces driving the evolution and biodiversity of double-stranded DNA viruses with large genomes. With the advent of comparative genomics, the phylogenetic relationships of baculoviruses have been put on solid bases.
Elisabeth A, Herniou, Johannes A, Jehle
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Baculovirus as Vaccine Vectors
Current Gene Therapy, 2010Application of viral vectors derived from human viruses to mediate immune response in animals and humans has been greatly hampered by the problems associated with pre-existing immunity and associated toxicities. Among few non-human viral vectors, baculovirus has now evolved as a novel tool for vaccine vector development.
Madhan, S., Prabakaran, M., Kwang, J.
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Structure of the baculovirus nucleocapsid
Virology, 1982A low-resolution structural model of the nucleocapsid of Spodoptera litura granulosis virus, a member of the Baculoviridae family, has been determined using contrast variation methods in both electron microscopy and low-angle X-ray solution scattering.
S K, Burley +3 more
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Baculovirus structural polypeptides
Virology, 1978Abstract The structural polypeptides of eight insect baculoviruses were studied using vertical slab polyacrylamide gel electrophoresis. All viruses revealed a complex but unique composition of 15 to 25 bands with molecular weights ranging from 15,000 to 160,000. Since certain baculoviruses have more than one nucleocapsid per viral envelope (multiples)
M D, Summers, G E, Smith
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Transfection with baculovirus DNA
Virology, 1980Purified DNA from the nuclear polyhedrosis viruses of Autographa californica (AcM NPV) and Rachiplusia ou (RoMNPV) were found to be infectious in TN-368 cells employing the calcium phosphate precipitation technique (F. L. Graham and A. J. van der Eb, Virology, 52 ,456-467, 1973).
J P, Burand, M D, Summers, G E, Smith
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