Results 221 to 230 of about 93,476 (270)

Fundamentals of Baculovirus Expression and Applications.

Advances in Experimental Medicine and Biology, 2016
In 1982 E. coli produced human insulin, the world's first recombinant DNA drug, was approved by the FDA. Since this historical event, remarkable progress has been made in developing bacterial, yeast, mammalian and insect cell protein expression systems that are used to produce recombinant proteins for both research and clinical applications.
T. Kost, C. Kemp
semanticscholar   +3 more sources

Bioprocessing of Baculovirus Vectors: A Review

Current Gene Therapy, 2010
The baculovirus/insect cell expression system is best known, and used, as a research tool for the production of recombinant proteins often requiring post-translational modifications. Although studies on the larger scale use of the system have been reported on for three decades, the recognition of this system as an industrially relevant platform for the
Aucoin, Marc G., Mena, Jimmy A., Kamen, Amine. A.   +2 more
openaire   +2 more sources

Replication of the Baculovirus Genome

Molecular Biology, 2003
The review describes the current state of studying the baculovirus DNA replication. The structural organization of replication initiation sites and replication intermediates are considered. Attention is focused on virus replication factors, including DNA polymerase, helicase, IE-1, LEF-1, LEF-2, and LEF-3.
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Manipulation of Baculovirus Vectors

2003
The development of expression vectors from baculoviruses has provided molecular biologists with one of the most efficient and effective ways of synthesizing foreign gene products in a heterologous system. In contrast to the other recent excellent reviews on the subject (1,2), this contribution aims to explore the various practical aspects of the system,
M J, Bailey, R D, Possee
openaire   +2 more sources

Introduction to Baculovirus Molecular Biology

2007
The development of baculovirus expression vector systems has accompanied a rapid expansion of our knowledge about the genes, their function, and regulation in insect cells. Classification of these viruses has also been refined as we learn more about differences in gene content between isolates, how this affects virus structure, and their replication in
Kelly, Barbara J., King, Linda A., Possee, Robert D.   +2 more
openaire   +4 more sources

Baculovirus Production and Infection in Axolotls

2022
Salamanders have served as an excellent model for developmental and tissue regeneration studies. While transgenic approaches are available for various salamander species, their long generation time and expensive maintenance have driven the development of alternative gene delivery methods for functional studies.
Prayag, Murawala, Catarina R, Oliveira, Helena, Okulski, Maximina H, Yun, Elly M, Tanaka   +4 more
openaire   +2 more sources

Construction of baculovirus recombinants

Cytotechnology, 1996
There are three main reasons for wishing to modify baculovirus genomes. The most common arises from the use of baculoviruses to express foreign genes. Baculovirus expression systems frequently give high level expression of proteins with full biological activity.
openaire   +2 more sources

Silkworm as a Host of Baculovirus Expression

Current Pharmaceutical Biotechnology, 2010
While the Baculovirus Expression Vector System (BEVS) mainly uses insect cell lines, such as Sf9 cells, the robust high expression system using silkworm has also been developed. We have further improved technologies for enhancement of virus recombination, reduction of proteolytic degradation and aggregation, and more reliable promoters.
Akihiro, Usami, Takeo, Suzuki, Hidekazu, Nagaya, Hiroki, Kaki, Seiji, Ishiyama   +4 more
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Specificity of Baculovirus p10 Functions

Virology, 1994
Three functional domains in baculovirus p10 proteins have been postulated for aggregation, nuclear disintegration, and fibrillar structure formation (Van Oers et al., J. Gen. Virol. 74, 563-574, 1993). To study the specificity of these functions, a recombinant Autographa californica nuclear polyhedrosis virus (AcCR1) was constructed in which the coding
van Oers, M.M., Flipsen, J.T.M., Reusken, C.B.E.M., Vlak, J.M.   +3 more
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The Baculovirus 10-kDa Protein

Journal of Invertebrate Pathology, 1997
The Baculoviridae are a family of rod-shaped (baculum 5 rod), enveloped, double-stranded DNA viruses with a circular, supercoiled genome varying in size between 90 and 160 kb (Murphy et al., 1995). The family is divided into two genera, nucleopolyhedrovirus (NPV) and granulovirus (GV). The virus replication cycle is biphasic resulting in the production
M M, Van Oers, J M, Vlak
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