Dynamic association of BAM complex modules includes surface exposure of the lipoprotein BamC. [PDF]
J Mol Biol, 2012 The β-barrel assembly machinery (BAM) complex drives the assembly of β-barrel proteins into the outer membrane of gram-negative bacteria. It is composed of five subunits: BamA, BamB, BamC, BamD, and BamE. We find that the BAM complex isolated from the outer membrane of Escherichia coli consists of a core complex of BamA:B:C:D:E and, in addition, a BamA:
Webb CT +5 more
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Structural basis of outer membrane protein insertion by the BAM complex [PDF]
Nature, 2016 All Gram-negative bacteria, mitochondria and chloroplasts have outer membrane proteins (OMPs) that perform many fundamental biological processes. The OMPs in Gram-negative bacteria are inserted and folded into the outer membrane by the β-barrel assembly machinery (BAM). The mechanism involved is poorly understood, owing to the absence of a structure of
Gu, Yinghong +10 more
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An In Vitro Assay for Outer Membrane Protein Assembly by the BAM Complex. [PDF]
Methods Mol Biol, 2015 To elucidate the mechanism of a biochemical process it is often essential to reconstitute the reaction in vitro using the minimal set of factors required to drive the reaction to completion. Here, we describe a method to reconstitute the folding and membrane integration of bacterial outer membrane (OM) proteins that have a characteristic β-barrel ...
Roman-Hernandez G, Bernstein HD.
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Assembly of Outer Membrane β-Barrel Proteins: the Bam Complex. [PDF]
EcoSal Plus, 2011 The major class of integral proteins found in the outer membrane (OM) of E. coli and Salmonella adopt a β-barrel conformation (OMPs). OMPs are synthesized in the cytoplasm with a typical signal sequence at the amino terminus, which directs them to the secretion machinery (SecYEG) located in ...
Malinverni JC, Silhavy TJ.
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Improper Coordination of BamA and BamD Results in Bam Complex Jamming by a Lipoprotein Substrate [PDF]
mBio, 2019 The β-barrel assembly machinery, the Bam complex, is central to the biogenesis of integral outer membrane proteins (OMPs) as well as OMP-dependent surface-exposed lipoproteins, such as regulator of capsule synthesis protein F (RcsF).
Muralidhar Tata, Anna Konovalova
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Discovery of a distinct BAM complex in the Bacteroidetes
AbstractThe BAM (β-barrel assembly machinery) complex is an evolutionarily conserved, multiprotein machine that catalyses the folding and membrane insertion of newly synthesised β-barrel outer membrane (OM) proteins in Gram-negative bacteria. Based on Proteobacteria, bacterial BAM is also structurally conserved, with an essential BamAD core and up to ...
Silale A +10 more
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The Synthetic Phenotype of Δ
mBio, 2019 The selective permeability of the Gram-negative outer membrane (OM) is maintained by integral β-barrel outer membrane proteins (OMPs). The heteropentomeric β-barrel assembly machine (Bam) folds and inserts OMPs into the OM.
Elizabeth M. Hart +3 more
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A modular BAM complex in the outer membrane of the alpha-proteobacterium Caulobacter crescentus. [PDF]
PLoS ONE, 2010 Mitochondria are organelles derived from an intracellular alpha-proteobacterium. The biogenesis of mitochondria relies on the assembly of beta-barrel proteins into the mitochondrial outer membrane, a process inherited from the bacterial ancestor ...
Khatira Anwari +11 more
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BB0324 and BB0028 are constituents of the
BMC Microbiology, 2012 Background Similar to Gram-negative bacteria, the outer membrane (OM) of the pathogenic spirochete, Borrelia burgdorferi, contains integral OM-spanning proteins (OMPs), as well as membrane-anchored lipoproteins.
Lenhart Tiffany R +4 more
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The evolution of new lipoprotein subunits of the bacterial outer membrane BAM complex. [PDF]
Mol Microbiol, 2012 SummaryThe β‐barrel assembly machine (BAM) complex is an essential feature of all bacteria with an outer membrane. The core subunit of the BAM complex is BamA and, in Escherichia coli, four lipoprotein subunits: BamB, BamC, BamD and BamE, also function in the BAM complex.
Anwari K +11 more
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