Results 41 to 50 of about 71,936 (305)

Spatial heterogeneity of hotspot mutations and clonal relatedness in ovarian endometrioid carcinoma

Molecular Oncology, EarlyView.
Spatially resolved analysis revealed heterogeneous distributions of selected cancer‐associated mutations within ovarian endometrioid carcinomas. Shared mutations between paired endometrial and ovarian tumors supported clonal relatedness. These findings highlight the value of sampling multiple tumor regions when interpreting molecular profiles ...
Takahito Ashihara, Kosuke Murakami, Shiro Takamatsu, Akiko Kanto, Seiichi Mori, Tomoyuki Otani, Kazuko Sakai, Yasushi Kotani, Kazuto Nishio, Kaoru Abiko, Noriomi Matsumura   +10 more
wiley   +1 more source

The impact of leukocyte‐associated RNA retention on platelet RNA‐based cancer detection

Molecular Oncology, EarlyView.
Platelets carry RNA signals that may help detect cancer, but platelet samples can also retain RNA from white blood cells. We found that this retained white blood cell signal did not significantly affect machine learning cancer classification, suggesting that the cancer‐related RNA signal in platelets is largely independent of leukocyte‐associated RNA ...
Michał Sieczczyński, Krzysztof Pastuszak, Anna Samelak‐Czajka, Zuzanna Cemka, Paulina Jackowiak, Tomasz Stefaniak, Sylwia Łapińska‐Szumczyk, Jacek Jassem, Anna J. Żaczek, Matthew T. Rondina, Myron G. Best, Sjors G. J. G. In 't Veld, Anna Supernat   +12 more
wiley   +1 more source

Mitochondrial cox1 sequence data reliably uncover patterns of insect diversity but suffer from high lineage-idiosyncratic error rates. [PDF]

, 2010
The demand for scientific biodiversity data is increasing, but taxonomic expertise is often limited or not available. DNA sequencing is a potential remedy to overcome this taxonomic impediment.
Ribera Ignacio, Hendrich, Lars, Pons, Joan, Balke, Michael, Ribera, Ignacio, Michael Balke, Balke Michael, Hendrich Lars, Lars Hendrich, Joan Pons, Pons Joan, Ignacio Ribera   +11 more
core   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Standardized nuclear markers improve and homogenize species delimitation in Metazoa

Methods in Ecology and Evolution, 2023
Species are the fundamental units of life and evolution. Their recognition is essential for science and society. Molecular methods have been increasingly used for the identification of animal species, despite several challenges.
Lars Dietz, Jonas Eberle, Christoph Mayer, Sandra Kukowka, Claudia Bohacz, Hannes Baur, Marianne Espeland, Bernhard A. Huber, Carl Hutter, Ximo Mengual, Ralph S. Peters, Miguel Vences, Thomas Wesener, Keith Willmott, Bernhard Misof, Oliver Niehuis, Dirk Ahrens   +16 more
doaj   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

VIP Barcoding: Composition vector-based software for rapid species identification based on DNA barcoding

, 2014
Species identification based on short sequences of DNA markers, that is, DNA barcoding, has emerged as an integral part of modern taxonomy. However, software for the analysis of large and multilocus barcoding data sets is scarce.
Fan, Long, Chu, Ka-Hou, Yu, Zuguo, Hui, Jerome   +3 more
core   +1 more source

Kaempferia chonburiensis (Zingiberaceae), a new species from Thailand based on morphological and molecular evidence [PDF]

PeerJ
Background Kaempferia is a genus belonging to the ginger family. Currently, this genus is comprised of about 63 species, mainly distributed from India to Southeast Asia.
Pornpimon Wongsuwan, Boonmee Phokham, Pantamith Rattanakrajang, Chayan Picheansoonthon, Suchada Sukrong   +4 more
doaj   +2 more sources

DNA Barcoding to Enhance Conservation of Sunshine Coast Heathlands

Diversity, 2022
Conservation priorities and decisions can be informed by understanding diversity patterns and the evolutionary history of ecosystems, and phylogenetic metrics can contribute to this.
Hilary Pearl, Tim Ryan, Marion Howard, Yoko Shimizu, Alison Shapcott   +4 more
doaj   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source