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Agrobacterium-Mediated Transformation of Barley (Hordeum vulgare L.)
2014Barley biotechnology requires efficient genetic engineering tools for producing transgenic plants necessary for conducting reverse genetics analyses in breeding and functional genomics research. Agrobacterium-mediated genetic transformation is an important technique for producing barley transgenics with simple low-copy number transgenes.
Ainur, Ismagul +3 more
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Ring chromosomes in barley (Hordeum vulgare L.)
Chromosoma, 1981A plant with 2n = 14 + 1 ring chromosomes was obtained in the progeny of a primary trisomie for chromosome 7 of a two-rowed cultivar, Shin Ebisu 16. The morphological characteristics of the trisomic plants with an extra ring chromosome were similar to the primary trisomic for chromosome 7 (Semierect), which suggests that it originated from this ...
R. J. Singh, T. Tsuchiya
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Barley (Hordeum vulgare L.) Transformation Using Immature Embryos
2014Barley is a major crop species, and also has become a genetic model for the small grain temperate cereals. A draft barley genome sequence has recently been completed, opening many opportunities for candidate gene isolation and functionality testing. Thanks to the development of customizable endonucleases, also site-directed genome modification recently
Cornelia, Marthe +2 more
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Advanced backcross QTL analysis in barley (Hordeum vulgare L.)
Theoretical and Applied Genetics, 2003This paper reports on the first advanced backcross-QTL (quantitative trait locus) project which utilizes spring barley as a model. A BC(2)F(2) population was derived from the initial cross Apex ( Hordeum vulgare ssp. vulgare, hereafter abbreviated with Hv) x ISR101-23 ( H. v. ssp. spontaneum, hereafter abbreviated with Hsp).
K, Pillen, A, Zacharias, J, Léon
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Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts
Plant Cell Reports, 1995Protoplasts isolated from calli derived from cultured microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar) were transformed with the neomycin phosphotransferase marker gene (nptII) by electroporation. Screening of the regenerated plants for the NPTII activity by gel assay resulted in three positive signals.
Salmenkallio-Marttila, Marjatta +8 more
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Somaclonal Variation in Barley (Hordeum vulgare L.)
1990Barley is the world’s fourth most important cereal crop after wheat, maize, and rice. Three unique characteristics have enabled barley to persist as a major cereal crop through many centuries: (1) broad ecological adaptation, (2) utility as a feed and food grain, and (3) superiority of barley malt for use in brewing.
A. Breiman, D. Rotem-Abarbanell
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FISH landmarks for barley chromosomes (Hordeum vulgare L.)
Genome, 1999Barley metaphase chromosomes (2n = 14) can be identified by fluorescence in situ hybridization (FISH) and digital imaging microscopy using heterologous 18S rDNA and 5S rDNA probe sequences. When these sequences are used together, FISH landmark signals were seen so that all 7 chromosomes were uniquely identified and unambiguously oriented.
S E, Brown +3 more
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Cytogenetics of four telotrisomics in barley (Hordeum vulgare L.)
Theoretical and Applied Genetics, 1990Four barley telotrisomics (Triplo 3S, 5S, 6S, and 7S) were studied. No major qualitative differences in morphology between the telotrisomics and their diploid sibs were found. The pollen and seed fertility of these telotrisomics was comparable to their diploid sibs.
S, Wang, T, Tsuchiya
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High Frequency Haploid Production in Barley (Hordeum vulgare L.)
Nature, 1970HAPLOIDS have been sought and investigated in many plant species since their first identification in Datura1. The chief limitation in using them to produce homozygous lines for plant breeding programmes or genetic research has been the lack of techniques by which large numbers of haploids can be obtained from any given stock2.
K J, Kasha, K N, Kao
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Transformation in Hordeum vulgare L. (Barley)
1993Genetic transformation of graminaceous plants has been mainly conducted by directly introducing foreign genes into their protoplasts by means of electroporation of polyethylene glycol treatment. In these methods, plant regeneration from protoplasts is a prerequisite for producing transgenic plants.
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