Results 31 to 40 of about 560,023 (258)

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

BODRUM-TORBA MONASTERY MOSAICS

Journal of Mosaic Research, 2009
Bodrum ilçesine bağlı Torba köyünde 2000 yılında yapılan kazılar sırasında Geç Roma Çağına tarihlenen taşınır ve taşınmaz eserler ortaya çıkarılmıştır. Tapınak-Şapel, Rahipler evi, Büyük Kilise, Hamam ve Sarnıç gibi taşınmaz eserler içinde yapılan kazı ...
Aykut ÖZET
doaj  

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Influence of Ultrasonic Bath on Mold-Assisted Electrodeposition of Gold Microelectrode Arrays

Proceedings
This study investigates the potential of ultrasonic baths to enhance mold-assisted electrodeposition for fabricating three-dimensional (3D) microelectrode arrays (MEAs) with improved quality and reliability.
Neeraj Yadav, Flavio Giacomozzi, Alessandro Cian, Damiano Giubertoni, Leandro Lorenzelli   +4 more
doaj   +1 more source

Biomechanical risk factors for knee osteoarthritis and lower back pain in lower limb amputees: protocol for a systematic review

BMJ Open, 2022
Introduction There is a limited research exploring biomechanical risk factors for the development of knee osteoarthritis (KOA) and lower back pain (LBP) between lower limb amputee subgroups, (eg, transtibial amputees (TTA) vs transfemoral amputees (TFA),
Carly McKay, Elena Seminati, James Bilzon, Logan Wade, M Polly McGuigan   +4 more
doaj   +1 more source

OCEAN BATHING. [PDF]

JAMA: The Journal of the American Medical Association, 1905
n ...
openaire   +2 more sources

Cell surface CD11c as a neutrophil aging marker molecule

FEBS Open Bio, EarlyView.
Cell surface CD11chi neutrophils were more aged and had better phagocytic function than CD11c−/lo neutrophils. Transcriptomic analysis of CD11chi neutrophils and CD11c−/lo neutrophils in pediatric population showed that the most difference was seen in infants.
Sophia Koutsogiannaki, Fahd Alhamdan, Erik Malm, Marie Bermudez, Samuel Y. Kim, Koichi Yuki   +5 more
wiley   +1 more source

Systemic evaluation on efficacy of chlorhexidine sponge bath for preventing catheterassociated urinary tract infections for adult patients in intensive care unit

Huli yanjiu, 2016
Objective:To evaluate the effect of daily chlorhexidine sponge bath for preventing catheterassociated urinary tract infections(CAUTI) in adult patients in the intensive care unit(ICU).Methods:Systematically searched PUBMED,EMBASE,the Cochrane Library and
黄华平, 陈斌, 王海燕
doaj