Results 131 to 140 of about 670,401 (315)
Evolution‐guided yeast complementation reveals functional differences in human PSPH variants
FEBS Open Bio, EarlyView.Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
Some Notes on Articles in Beads [PDF]
Some Notes on Articles in Beads, by Peter Francis, Jr.
Francis, Jr., Peter
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Adenosine triphosphate as a modulator of protein interactions and stability
FEBS Open Bio, EarlyView.ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
More on Fustat Fused Rod Beads [PDF]
More on Fustat Fused Rod Beads, by Peter Francis, Jr.
Francis, Jr., Peter
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FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
European Trade Beads in Southern Africa [PDF]
European Trade Beads In Southern Africa, By David Killick (1987, 10:3 ...
Killick, David
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Trade Beads From Hudson\u27s Bay Company Fort Vancouver (1829-1860), Vancouver, Washington [PDF]
, 1990 Archaeological excavations conducted at Hudson\u27s Bay Company Fort Vancouver recovered 100,000+ trade beads of 152 varieties, including 80 varieties of drawn, 57 varieties of wound, 10 varieties of mold-pressed and 3 varieties of blown glass beads, as ...
Ross, Lester A.
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Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Eighteenth-century Chevron Beads in the Southeast [PDF]
Eighteenth-Century Chevron Beads In The Southeast, By Marvin T.
Smith, Marvin T.
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