Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Psychological, psychiatric, and behavioral sciences measurement scales: best practice guidelines for their development and validation. [PDF]
Stefana A +6 more
europepmc +1 more source
Early‐life exposure to a high‐fat diet altered intact Achilles tendons in rat offspring, making them thinner, stiffer, and molecularly distinct even without injury. These findings suggest that developmental high‐fat diet exposure may impair tendon quality and increase susceptibility to mechanical overload or tendon injury later in life.
Heyong Yin +3 more
wiley +1 more source
Correction: Pohlman et al. (2025). Interconnecting District and Community Partners to Improve School-Level Social, Emotional, and Behavioral Health. <i>Behavioral Sciences</i>, <i>15</i>(9), 1225. [PDF]
Pohlman KB +4 more
europepmc +1 more source
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata +13 more
wiley +1 more source
Correction: Liu et al. (2025). Emotion Regulation Modulates Affective Responses Without Altering Memory Traces: A Study of Negative Social Feedback from Acquaintances. <i>Behavioral Sciences</i>, <i>15</i>(9), 1294. [PDF]
Liu P, Cheng X, Fan M, Huang Z, Zhang C.
europepmc +1 more source
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová +2 more
wiley +1 more source
Editorial: Extended mind for the design of human environment
Massimo Angrilli +6 more
doaj +1 more source
The current practice of latent growth curve modeling in the social and behavioral sciences: Observations and Recommendations. [PDF]
Miller ML, Ferrer E, Ghisletta P.
europepmc +1 more source

