Results 31 to 40 of about 19,244 (227)

Synthesis of Six Natural Products Enabled by Selective Hydroxylation of Orcinaldehyde‐Like Substrates With an Enzyme Library

Angewandte Chemie, EarlyView.
Achieving selective (sp3)C–H hydroxylation can streamline the synthesis of complex organic molecules. Biocatalysis presents an opportunity to realize this transformation, however, identifying useful enzymes that enable synthetic strategies can be a challenge due to limitations in enzyme substrate scope.
Gonzalo J. Villegas Rodríguez, Evan O. Romero, Jonathan C. Perkins, Diego N. Rojas, Maximilian S. Hrigora, Alison R. H. Narayan   +5 more
wiley   +2 more sources

An efficient and regioselective biocatalytic synthesis of aromatic N‐oxides by using a soluble di‐iron monooxygenase PmlABCDEF produced in the Pseudomonas species

Microbial Biotechnology, 2021
Summary Here, we present an improved whole‐cell biocatalysis system for the synthesis of heteroaromatic N‐oxides based on the production of a soluble di‐iron monooxygenase PmlABCDEF in Pseudomonas sp. MIL9 and Pseudomonas putida KT2440.
Vytautas Petkevičius, Justas Vaitekūnas, Renata Gasparavičiūtė, Daiva Tauraitė, Rolandas Meškys   +4 more
doaj   +1 more source

Engineering Microbial Particles for Next‐Generation Biomedical Platforms

Advanced Science, EarlyView.
Microbe‐derived particles (MDPs), which include extracellular vesicles, outer membrane vesicles, inclusion bodies, polysaccharide particles, and virus‐like particles, represent a rapidly expanding category of bioinspired nanomaterials. With their natural origin, intrinsic biocompatibility, and highly programmable functionality, MDPs serve as a ...
Yuting Li, Yunjie Lu, Ziyan Huang, Jianjiang Chen, Yanbin Liu, Lei Qin, Chao Wang, Yumin Wu   +7 more
wiley   +1 more source

Understanding the Catalytic Determinant role of Diaphorase‐Like Subunit in Formate Dehydrogenases via Redox Couples

Advanced Science, EarlyView.
A unique mechanism of catalytic bias regulated by diaphorase‐like subunit in formate dehydrogenase from Rhodobacter aestuarii is revealed. The diaphorase‐like subunit functions act as a biological “voltage rheostat” that controls the slow release of NADH to regulate redox balance, biasing the enzyme's catalytic preference toward CO2 reduction over ...
Kuncheng Zhang, Weisong Liu, Hao Su, Huijuan Cui, Yuanming Wang, Zhiguang Zhu, Chun You, Lingling Zhang   +7 more
wiley   +1 more source

Amidase‐Catalyzed Desorption of CO2 Captured in Aqueous Monoethanolamine (MEA) Solutions

Angewandte Chemie, EarlyView.
CO2 is captured in aqueous monoethanolamine (MEA) sorbents as carbamate and bicarbonate/carbonate. The subsequent desorption for storage or utilization is energy‐intensive, and this is a major impediment to industrial carbon dioxide removal (CDR) processes. The desorption process can be promoted by enzymes that catalyze the dissociation of distinct CO2‐
Yang Yang, Onur Kırtel, Silke Flindt Badino, Lea Helena Strother, Laura Rotilio, Jerik Mathew Valera Lauridsen, Stefanie Neun, Jens Preben Morth, Ji‐Woong Lee, Ditte Hededam Welner, Peter Westh   +10 more
wiley   +2 more sources

Protein Structuromics Reveals a Loop‐Controlled Half‐Open Active Pocket Conformation Throughout Fe(II)/α‐ketoglutarate‐Dependent Dioxygenase Catalytic Cycle

Advanced Science, EarlyView.
This study reveals a conserved half‐open active pocket in Fe(II)/α‐ketoglutarate‐dependent dioxygenases through protein structuromics, distinguished by structural features comprising highly flexible loop elements. Enhanced sampling simulations‐guided mutagenesis experiments elucidated how this flexible loop regulates pocket open/closed conformations ...
Lunjie Wu, Huan Liu, Songyin Zhao, Lei Qin, Jiarui Li, Jun Wang, Zixuan Dai, Jie Gu, Yan Xu, Feiran Li, Yao Nie   +10 more
wiley   +1 more source

Environmental Identification of Novel Enzymes for Polyurethane and Polyamide Degradation

Angewandte Chemie, EarlyView.
Heteroatom‐containing plastics such as polyurethanes and nylons are produced at scale yet remain largely unrecycled. Biocatalysts offer a promising route to depolymerization under mild conditions. Here, we present a screening platform for the discovery of novel polyurethane‐ and nylon‐degrading enzymes from environmental samples and demonstrate ...
Malthe Kjær Bendtsen, Andreas Møllebjerg, Samuel Peña‐Díaz, Rosie Graham, Nicolai Claus Petersen, Bjørk Nolsøe Isaksen, Mathias Carstensen, Martin B. Johansen, Andreas Sommerfeldt, Allan R. Petersen, Iddi Khamisi Chuma, Cecilie Ryberg, Thomas Rea Wittenborn, Virginia Gichuru, Huabing Wang, Carsten Scavenius, Alexander Sandahl, Daniel E. Otzen   +17 more
wiley   +2 more sources

Biocatalysis for Biobased Chemicals

Biomolecules, 2013
The design and development of greener processes that are safe and friendly is an irreversible trend that is driven by sustainable and economic issues.
Rubén de Regil, Georgina Sandoval
doaj   +1 more source

IRES-mediated Pichia pastoris cell-free protein synthesis

Bioresources and Bioprocessing, 2023
Cell-free protein synthesis (CFPS) system is an ideal platform for fast and convenient protein research and has been used for macromolecular assembly, unnatural amino acid embedding, glycoprotein production, and more.
Yanan Wang, Ting Wang, Xinjie Chen, Yuan Lu   +3 more
doaj   +1 more source

Mechanistic Understanding of Protein–MOF Integration Through Surfactant‐Driven Interfacial Design

Advanced Science, EarlyView.
This study reveals how surfactant‐driven interfacial design governs the assembly and stability of protein@MOF composites. Using lipid‐based nonionic surfactants, we modulate protein–MOF interactions to improve encapsulation efficiency, MOF crystallization, and catalytic performance.
Ehsan Rashidniyaghi, Mohammad Khavani, Carlie Coerver, Ruibin Liang, Raheleh Ravanfar   +4 more
wiley   +1 more source