The impact of leukocyte‐associated RNA retention on platelet RNA‐based cancer detection
Molecular Oncology, EarlyView.Platelets carry RNA signals that may help detect cancer, but platelet samples can also retain RNA from white blood cells. We found that this retained white blood cell signal did not significantly affect machine learning cancer classification, suggesting that the cancer‐related RNA signal in platelets is largely independent of leukocyte‐associated RNA ...
Michał Sieczczyński +12 more
wiley +1 more source
Probing human immune cell mechanics using acoustic force spectroscopy
STAR ProtocolsSummary: Immune cells continuously adapt their mechanical properties for proper circulation and elicitation of immune responses. Here, we provide a step-by-step protocol for probing the single-cell mechanical properties of primary human monocytes using ...
Tom M.J. Evers +2 more
doaj +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Frontiers in Bioengineering and Biotechnology: Plant Nanoparticles for Anti-Cancer Therapy. [PDF]
Vaccines (Basel), 2021 Shoeb E +3 more
europepmc +1 more source
Thesis (Master)--İzmir Institute of Technology: Biotechnology and Bioengineering.
, 2005 Thesis (Master)--İzmir Institute of Technology, Biotechnology and Bioengineering, İzmir, 2005Includes bibliographical references (leaves: 88-101)Text in English; Abstract: Turkish and Englishxiii, 104 leavesIn this study, partially purified lyophilized ...
Mecitoğlu Güçbilmez, Çiğdem
core
The Impact Of Consumer Attitudes About Bioengineering On Purchase Behavior Intentions: Moderating Effects Of Innate Consumer Innovativeness And Anxiety [PDF]
, 2004 Biotechnology is anticipated to be the next generation of innovations and is predicted to surpass the IT revolution in terms of economic prosperity (Darby and Zucher, 2003). But available behavioral and social research relating to biotechnology is ...
Guha, Gauri S. +2 more
core +1 more source
Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids
FEBS Open Bio, EarlyView.Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão +3 more
wiley +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
, 2010 Polycrystalline alumina ceramic orthodontic brackets were coated with anatase TiO 2 film via a sol-gel dip-coating method. The surface structure morphology and composition of the films were evaluated via scanning electron microscopy, atomic force ...
Uzel A. +5 more
core +1 more source