Results 121 to 130 of about 32,871 (300)

Optimizing Pichia Pastoris Cell‐Free Protein Synthesis to Improve Economics

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Cell‐free protein synthesis (CFPS) is a powerful and versatile platform that supports a wide range of applications, from fundamental studies of the genetic code to scalable and rapid protein production. The recently developed Pichia pastoris CFPS combines advantages of both prokaryotic and eukaryotic systems, including a rapid growth rate ...
Rui Wu, Igor Koop, Gavin Murugan, Thagshanen Thillairajah, Karen M. Polizzi   +4 more
wiley   +1 more source

Addressing Small Data Challenges in Biopharmaceutical Development and Manufacturing: A Mini Review of Multi‐Fidelity Techniques

Biotechnology and Bioengineering, EarlyView.
ABSTRACT The growing demand for biopharmaceutical products reflects their effectiveness in medical treatments. However, developing new biopharmaceuticals remains a major bottleneck, often taking up to a decade before market approval. Machine learning (ML) models have the potential to accelerate this process, but their success depends on access to large
Mohammad Golzarijalal, Uwe Aickelin, Ellen Otte   +2 more
wiley   +1 more source

Physical properties and solubility studies of Nifedipine-PEG 1450/HPMCAS-HF solid dispersions [PDF]

, 2018
Low-order high-energy nifedipine (NIF) solid dispersions (SDs) were generated by melt solvent amorphization with polyethylene glycol (PEG) 1450 and hypromellose acetate succinate (HPMCAS-HF) to increase NIF solubility while achieving acceptable physical ...
Chougule, Mahavir B., Dave, Vivek S, Gavireddi, Monika, Gupta, Deepak, Haware, Rahul V., Stagner, William C, Vinjamuri, Bhavani Prasad   +6 more
core   +1 more source

Nicotinamide Reverses the Warburg Effect in CHO Cell Culture

Biotechnology and Bioengineering, EarlyView.
Abstract The Warburg effect, the preferential conversion of glucose‐derived pyruvat to lactate despite available oxygen, is a key feature of Chinese hamster ovary (CHO) cell culture. Lactate accumulation in recombinant protein‐producing cell culture is an inefficient usage of glucose, as well as being deleterious to cells.
James Morrissey, Ayca Cankorur‐Cetinkaya, Luigi Grassi, Annie J. Harwood‐Stamper, Jonathan Welsh, Ryte Poskute, Kasia Kozakowska‐McDonnell, Cleo Kontoravdi   +7 more
wiley   +1 more source

Inhalation of Immuno-Therapeutics/-Prophylactics to Fight Respiratory Tract Infections: An Appropriate Drug at the Right Place!

Frontiers in Immunology, 2019
Thomas Sécher, Thomas Sécher, Alexie Mayor, Alexie Mayor, Nathalie Heuzé-Vourc'h, Nathalie Heuzé-Vourc'h   +5 more
doaj   +1 more source

Improving activity of GenB3 and GenB4 in gentamicin dideoxygenation biosynthesis by semi-rational engineering

Microbial Cell Factories
Background Aminoglycoside antibiotics continue to play an indispensable role in clinical antibacterial agents. However, the protection and deprotection procedures in the chemical pathways of semi-synthetic antibiotics are long, atom- and step-inefficient,
Hang Zhai, Lihua Yang, Qi Ye, Zhijun Kong, Jiye Pei, Yuan Ji, Botong Liu, Xiaotang Chen, Tingting Tian, Xianpu Ni, Huanzhang Xia, Shumin Zhang   +11 more
doaj   +1 more source

Regional variation in the gut microbiota of olive flounder (Paralichthys olivaceus) farmed in Korea

Fisheries and Aquatic Sciences
The gut microbiota composition is closely linked to fish health. Understanding this composition may help develop strategies to enhance the productivity and sustainability of aquaculture. In this study, we investigated the gut microbiota of olive flounder
Yu-Ri Kim, Young-Sun Lee, Su-Jeong Lee, Soo-Bin Hwang, Won Je Jang, Eun-Woo Lee   +5 more
doaj   +1 more source

Advancing design strategies in smart stimulus‐responsive liposomes for drug release and nanomedicine

BMEMat, EarlyView.
Schematic illustration of stimulus‐responsive liposomes designed for controlled drug release and nanomedicine. The innermost circle represents different liposomal structures, including unilamellar, multilamellar, and multivesicular liposomes. The middle layer illustrates the responsive phospholipid components.
Yuchen Guo, Nan Li, Daiwei Zhang, Jingya Gu, Zhihuan Liao, Zihao Teng, Xiangfu Du, Peter S. Timashev, Shipeng Chen, Shuaidong Huo   +9 more
wiley   +1 more source

Microtopography enhances intestinal drug absorption via Piezo1‐mediated tight junction modulation

BMEMat, EarlyView.
Microtopography‐driven enhancement of oral peptide drug delivery by activating Piezo1 channels significantly improves paracellular permeability and bioavailability, offering a novel approach for optimizing macromolecule absorption in pharmaceutical formulations.
Yu Hu, Nan Xu, Xin Li, Xiaowo Kang, Tieyuan Bian, Junsheng Lin, Hao Tang, Yinyin Bao, Lu Zhang, Hongyan Yuan, Mingxi Yao, Xingyu Jiang, Zhi Luo   +12 more
wiley   +1 more source

Marine silicon for biomedical sustainability

BMEMat, EarlyView.
Schematic illustrating marine silicon for biomedical engineering. Abstract Despite momentous divergence from oceanic origin, human beings and marine organisms exhibit elemental homology through silicon utilization. Notably, silicon serves as a critical constituent in multiple biomedical processes.
Yahui Han, Zhibo Yang, Wei Xia, Chengtie Wu   +3 more
wiley   +1 more source