Results 191 to 200 of about 39,009 (272)

Adherent expansion of CHO-K1 and HEK-293 cells in a rotating polylactic-acid bioreactor: a scalable bioprocess^. [PDF]

MethodsX
Watzl F, Eichberg J, Herchenröder A, Günther K, Käßer L, Boshof B.   +5 more
europepmc   +1 more source

In Situ Imaging for Monitoring Biomass Content and Pellet Size in Submerged Filamentous Stirred‐Tank Cultivation

Biotechnology Journal, Volume 21, Issue 10, October 2026.
Graphical Abstract and Lay Summary Noninvasive imaging through a stirred‐tank reactor wall enabled time‐resolved monitoring of biomass and projected pellet size. Model‐sphere validation identified size‐ and concentration‐dependent biases; in Aspergillus oryzae cultivation, luminosity followed biomass, pellet‐size increases coincided with Zn2 ...
Lukas Hartmann, Tarek Wieland, Linus Witucki, Mike Barth, Dirk Holtmann   +4 more
wiley   +1 more source

Variable-specific preprocessing enables cross-scale transferable Raman models from high-throughput bioreactor data. [PDF]

Bioresour Bioprocess
Han SH, Park J, Lee KB, Park CH, Lee DY.
europepmc   +1 more source

Model‐Enabled Knowledge Transfer Across Cell Lines, Culture Scales and Conditions

Biotechnology and Bioengineering, Volume 123, Issue 10, Page 2613-2628, October 2026.
ABSTRACT Mechanistic models are central to quantitative understanding and optimization of Chinese hamster ovary (CHO) cell culture processes, but their utility is often restricted by parameter sets calibrated for specific cell lines, scales, or operating conditions.
Luxi Yu, Antonio del Rio Chanona, Cleo Kontoravdi   +2 more
wiley   +1 more source

Expanding Transposase Technology to Regulatory Noncoding RNAs for Stable Expression in CHO Cells. [PDF]

Biotechnol J
Lochmueller S, Weiss L, Otte K.
europepmc   +1 more source

Spray‐Dried Engineered Escherichia coli Whole‐Cell Biocatalysts Enable Cell Recycling and In Vivo α‐Ketoglutarate and NADPH Regeneration for Efficient Codeine Manufacture

Biotechnology and Bioengineering, Volume 123, Issue 10, Page 2629-2642, October 2026.
ABSTRACT A recombinant whole‐cell biocatalytic approach provides several advantages over isolated enzymes, such as improved enzyme stability, in vivo cofactor regeneration, and the facilitation of cascade reactions within a single cell. Whole‐cell biocatalysis has gained attention in medicinal opioid production, due to its technical and economic ...
Ali Jahanian, Carlos Horacio Luna‐Flores, Xu Li, Sally L. Gras, Robert E. Speight   +4 more
wiley   +1 more source

Biosynthesis of cyanophycin by high-cell-density cultivation of recombinant Escherichia coli for corrosion-resistant biomaterial applications. [PDF]

Bioprocess Biosyst Eng
Kafle SR, Mukunth A, Han A, Jayaraman A, Aristidou A.   +4 more
europepmc   +1 more source

Metabolic Flux Analysis Reveals Cell Line‐Specific Rewiring in CHO Cells Following TCA Cycle Intermediate Feeding for Bioprocess pH Control

Biotechnology and Bioengineering, Volume 123, Issue 10, Page 2713-2728, October 2026.
ABSTRACT Chinese hamster ovary (CHO) cells are the leading host for recombinant therapeutic protein production in the biopharma industry. In this study, we investigated how feeding acidic forms of tricarboxylic acid (TCA) cycle intermediates—malic acid, succinic acid, and α‐ketoglutaric acid—affects cell culture performance and metabolism in two ...
Sarah A. Sacco, Kevin J. Ruiz‐Márquez, Rachel Moen, Irina Trenary, Xiaolin Zhang, Xiaowen Wang, Malik Padellan, Henry Lin, Jamey D. Young   +8 more
wiley   +1 more source

A Guide to Bayesian Optimization in Bioprocess Engineering. [PDF]

Biotechnol Bioeng
Siska M, Pajak E, Rosenthal K, Del Rio Chanona A, von Lieres E, Helleckes LM.   +5 more
europepmc   +1 more source

Translating Blue Light Stimulation From Batch to Perfusion: Process and Intracellular Metabolic Analysis

Biotechnology and Bioengineering, Volume 123, Issue 10, Page 2729-2746, October 2026.
ABSTRACT Improving cell‐specific productivity remains a central objective in biopharmaceutical manufacturing. In this study, the effect of blue light illumination on IgG1‐producing CHO DP‐12 cells was systematically evaluated across batch, fed‐batch, and perfusion cultivations in controlled 3 L bioreactor systems.
Stefanie Föller, Attila Teleki, Ralf Takors   +2 more
wiley   +1 more source