Anchorage‐independent and faster growth in clonal population from UV‐irradiated NER‐deficient cells
UV‐irradiated cells expressing a DDB2 mutant protein unable to interact with PCNA (DDB2PCNA‐) form clones able to grow without anchorage. Different experimental approaches reveal heterogeneity in cell cycle regulation and drug response within these clones, emphasizing the crucial role of the DDB2‐PCNA interaction in preventing cellular transformation ...
Paola Perucca +6 more
wiley +1 more source
Photonic Crystal-Based Sensing and Imaging of Potassium Ions
We report on a method for selective optical sensing and imaging of potassium ions using a sandwich assembly composed of layers of photonic crystals and an ion-selective membrane.
Christoph Fenzl +3 more
doaj +1 more source
Comparing the fundamental limit of detection for interferometric and resonant biosensors with coherent phase read-out [PDF]
We compare the limit of detection of coherently interrograted photonic biosensors, using both interferometric and resonant architectures.Universidad de Málaga.
Halir, Robert +4 more
core +1 more source
Development of human monoclonal antibodies against TARM1 by yeast display
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe +5 more
wiley +1 more source
On the Physical Design of Molecular Communication Receiver Based on Nanoscale Biosensors
Molecular communications (MC), where molecules are used to encode, transmit, and receive information, is a promising means of enabling the coordination of nanoscale devices.
Akan, Ozgur B., Kuscu, Murat
core +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
ABSTRACT Background X‐linked adrenoleukodystrophy (X‐ALD) is a neurometabolic disorder caused by pathogenic variants in ABCD1, leading to slowly progressive spinal cord disease in nearly all affected men. Sensitive biomarkers to quantify disease severity and predict progression are needed for clinical care and trial design.
Eda G. Kabak +4 more
wiley +1 more source
Two-Dimensional Carbon Nanomaterials for Electrochemical and Plasmonic Sensing Applications
Two-dimensional carbon nanomaterials are very popular in chemo- and biosensor development. [...]
Eva-Maria Kirchner +3 more
doaj +1 more source
Whole-cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production [PDF]
Many high-value added recombinant proteins, such as therapeutic glycoproteins, are produced using mammalian cell cultures. In order to optimise the productivity of these cultures it is important to monitor cellular metabolism, for example the utilisation
Ainsworth, C +5 more
core +1 more source
Harnessing Fungal Biowelding for Constructing Mycelium‐Engineered Materials
Mycelium‐bound composites (MBCs) offer low‐carbon alternatives for construction, yet interfacial bonding remains a critical challenge. This review examines fungal biowelding as a biocompatible adhesive, elucidating mycelium‐mediated interfacial mechanisms and their role in material assembly. Strategies to optimize biowelding are discussed, highlighting
Xue Brenda Bai +2 more
wiley +1 more source

