Results 81 to 90 of about 202,591 (301)

Cloning and characterization of beta‐lactam biosynthetic genes [PDF]

Molecular Microbiology, 1989
SummarySeven genes coding for two different enzymes of the penicillin/cephalosporin biosynthetic pathway have been cloned from fungal and bacterial sources. Using amino acid sequences derived from the purified enzymes, oligonucleotide probes were designed to hybridize to their cognate genes in a genomic library.
J R, Miller, T D, Ingolia
openaire   +2 more sources

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source

Evolution and Diversity of Biosynthetic Gene Clusters in Fusarium

Frontiers in Microbiology, 2018
Plant pathogenic fungi in the Fusarium genus cause severe damage to crops, resulting in great financial losses and health hazards. Specialized metabolites synthesized by these fungi are known to play key roles in the infection process, and to provide survival advantages inside and outside the host.
Koen Hoogendoorn, Koen Hoogendoorn, Lena Barra, Cees Waalwijk, Jeroen S. Dickschat, Theo A. J. van der Lee, Marnix H. Medema   +6 more
openaire   +4 more sources

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

Diversity analysis of Streptomycetes and associated phosphotranspherase genes in soil [PDF]

, 2012
An attempt was made to verify the observation that Streptomyces griseus was prevalent in soil based on isolation work. A genus-specific PCR was developed for Streptomyces based on the housekeeping gene atpD and used to investigate species diversity ...
Paris Laskaris, Takuya Sekine, Laskaris Paris, Sekine Takuya, Elizabeth M H Wellington, Sekine, Takuya, Wellington, E. M. H., Laskaris, Paris, Wellington Elizabeth M. H.   +8 more
core   +1 more source

Interorgan regulation of ethylene biosynthetic genes by pollination. [PDF]

The Plant Cell, 1993
Pollination initiates a syndrome of developmental events that contribute to successful reproduction, including perianth senescence, changes in pigmentation, and ovule differentiation in preparation for impending fertilization. In orchid flowers, initiation of each of these processes in distinct floral organs is strictly and coordinately controlled by ...
S D, O'Neill, J A, Nadeau, X S, Zhang, A Q, Bui, A H, Halevy   +4 more
openaire   +2 more sources

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Metabologenomic profiling of the endemic Australian fungus Aspergillus luteorubrus

Mycology
Novel and rare fungal species continue to serve as an invaluable source of new natural products. In this study, we conducted a comprehensive metabologenomic assessment of the endemic Australian fungus Aspergillus luteorubrus MST-FP2246. Genome sequencing
Amr A. Arishi, Nirmal K. Chaudhary, John A. Kalaitzis, Daniel Vuong, Andrew Crombie, Cameron L. M. Gilchrist, Ernest Lacey, Peter Karuso, Andrew M. Piggott, Yit-Heng Chooi   +9 more
doaj   +1 more source

Bioprospecting Red Sea Coastal Ecosystems for Culturable Microorganisms and Their Antimicrobial Potential

Marine Drugs, 2016
Microorganisms that inhabit unchartered unique soil such as in the highly saline and hot Red Sea lagoons on the Saudi Arabian coastline, represent untapped sources of potentially new bioactive compounds.
Soha Al-Amoudi, Magbubah Essack, Marta F. Simões, Salim Bougouffa, Irina Soloviev, John A. C. Archer, Feras F. Lafi, Vladimir B. Bajic   +7 more
doaj   +1 more source

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

Molecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano, Giuseppina Minopoli, Simona Romano, Laura Marrone, Katia Aquilano, Maria Lina Tornesello, Raffaella Faraonio   +6 more
wiley   +1 more source