Results 51 to 60 of about 3,376 (150)
Purification of mRNA with oligo(dT)‐functionalized magnetic particles involves a series of magnetic separations for buffer exchange and washing. Magnetic particles interact and agglomerate with each other when a magnetic field is applied, which can ...
Lars Wommer +3 more
doaj +1 more source
ABSTRACT Diabetes mellitus is a chronic metabolic disorder characterized by the loss or dysfunction of insulin‐producing beta (β) cells. Adipose‐derived stem cells (ADSCs) represent a promising source for generating functional insulin‐producing β cells due to their accessibility and differentiation potential.
O. Daramola, H. Abrahamse, A. Crous
wiley +1 more source
Abstract BACKGROUND Adeno‐Associated Virus vectors are widely used in gene therapy due to their safety and efficacy. Despite the interest in their therapeutical application, AAV purification remains a significant challenge. Density gradient ultracentrifugation using cesium chloride, sucrose, or iodixanol gradients are common methods for AAV ...
Sabrina Leigheb +6 more
wiley +1 more source
ABSTRACT Advances in non‐invasive genetic sampling and long‐term genetic monitoring programmes have enabled collection of large individual genotype datasets for many wildlife populations, often accompanied by rich field metadata that place the genotyped individuals in time and space.
Gregor Simčič, Tomaž Skrbinšek
wiley +1 more source
SOIL EXAMINATION AND RECOMMENDATIONS FOR CREATING BETTER CONDITIONS FOR RASPBERRY PLANTING
When choosing land for raising a new planting of vegetables or fruit crops, the composition of the soil plays an important role; therefore, the composition of soil is examined, and chemical analysis of soil is made.
Jovica MOMIRCESKI +2 more
doaj
The widespread use of synthetic pesticides has raised concerns about their impacts on soil biodiversity and ecosystem functioning, increasing interest in plant-derived compounds as environmentally compatible alternatives. Cannabis sativa L.
Žiga Laznik +4 more
doaj +1 more source
Subterranean environments contribute to three‐quarters of classified ecosystem services
ABSTRACT Beneath the Earth's surface lies a network of interconnected caves, voids, and systems of fissures forming in rocks of sedimentary, igneous, or metamorphic origin. Although largely inaccessible to humans, this hidden realm supports and regulates services critical to ecological health and human well‐being.
Stefano Mammola +30 more
wiley +1 more source
Functional thermodynamics govern the ligand binding to human cytosolic transport proteins
Abstract Traditional binding studies on cellular, molecular transporters often simplify the thermodynamics of binding processes. We present a method that combines spin probing, electron paramagnetic resonance (EPR) spectroscopy and spectral simulations with binding studies and thermodynamics for an in‐depth molecular view into ligand binding and ...
Sebastian Michler +3 more
wiley +1 more source
ABSTRACT Research based on farmer surveys is a cornerstone of agricultural economics. Farmer surveys provide unique insights into behavioural variables—such as values, motivations, attitudes, behaviours, and preferences—that are unavailable in secondary datasets.
Thomas Slijper +24 more
wiley +1 more source
INTRODUCTION: Paper currency notes, exchangeable fomite, that is continuously contaminated because of the poor handling and storage practices. Objective: the general objective of the study was to determine the bacterial contamination of paper currency ...
Muhumuza Allan +4 more
doaj +1 more source

