Results 21 to 30 of about 102,201 (258)

Genetic modification, characterization, and co-infection of Franken Sphingomonas and Anaplasma phagocytophilum in tick cells

STAR Protocols, 2023
Summary: Paratransgenesis through genetic manipulation of symbiotic or commensal microorganisms has been proposed as an effective and environmentally sound approach for the control of vector-borne diseases, including tick bite-related pathologies, and ...
Lorena Mazuecos, Almudena González-García, José de la Fuente   +2 more
doaj   +1 more source

Editorial: Bioengineering and Biotechnology Approaches in Cardiovascular Sciences

Frontiers in Bioengineering and Biotechnology, 2021
Martin L Tomov, Jianyi Zhang, James Chong   +2 more
exaly   +3 more sources

Fabricating self-powered microfluidic devices via 3D printing for manipulating fluid flow

STAR Protocols, 2022
Summary: Advances in 3D printing technologies allow fabrication of complex structures at micron resolution. Here, we describe two approaches of fabricating self-powered microfluidic devices utilizing 3D printing: PDMS (polydimethylsiloxane)-based ...
Sung Oh Woo, Myungkeun Oh, Yongki Choi
doaj   +1 more source

Inducing T cell dysfunction by chronic stimulation of CAR-engineered T cells targeting cancer cells in suspension cultures

STAR Protocols, 2023
Summary: Several pre-clinical models reveal that chronic chimeric antigen receptor (CAR) stimulation drives a dysfunctional state that mimics in vivo failure.
Mehmet Emrah Selli, Jack H. Landmann, Corvin Arveseth, Nathan Singh   +3 more
doaj   +1 more source

Protocol to engineer nanofilms embedded lipid nanoparticles for controlled and targeted drug delivery (NECTAR)

STAR Protocols, 2023
Summary: We present a protocol to engineer a substrate-mediated delivery platform comprising hyaluronic acid-coated lipid nanoparticles (HALNPs) embedded into polyelectrolyte multilayer (PEM) films.
Rashi Porwal, Stephen L. Hayward, Srivatsan Kidambi   +2 more
doaj   +1 more source

Protocol to combine brain sections from multiple mice into a single block for spatial transcriptomic analyses

STAR Protocols, 2023
Summary: Spatial transcriptomics couples visual spatial markers with gene expression levels, but slide space and cost limit the number of samples that can be processed.
Yoshitaka J. Sei, Myriam M. Chaumeil, Ken Nakamura   +2 more
doaj   +1 more source

A protocol to execute a lab-on-chip platform for simultaneous culture and electrochemical detection of bacteria

STAR Protocols, 2023
Summary: Here, we present a protocol for a miniaturized microfluidic device that enables quantitative tracking of bacterial growth. We describe steps for fabricating a screen-printed electrode, a laser-induced graphene heater, and a microfluidic device ...
Sonal Fande, Sangam Srikanth, Jayapiriya U S, Khairunnisa Amreen, Satish Kumar Dubey, Arshad Javed, Sanket Goel   +6 more
doaj   +1 more source

Enzyme-linked immunosorbent assay to detect surface marker proteins of extracellular vesicles purified from human urine

STAR Protocols, 2023
Summary: The molecular profile of extracellular vesicles released in urine reflects the pathophysiological processes occurring within originating cells located in diverse nephron segments.
Keiichi Takizawa, Tatsuya Nishimura, Yutaka Harita   +2 more
doaj   +1 more source

Using EuGeneCiD and EuGeneCiM computational tools for synthetic biology

STAR Protocols, 2021
Summary: Synthetic biology often relies on the design of genetic circuits, utilizing “bioparts” (modular DNA pieces) to accomplish desired responses to external stimuli.
Wheaton L. Schroeder, Anna S. Baber, Rajib Saha   +2 more
doaj   +1 more source

Biotechnology and Bioengineering [PDF]

, 2019
Biotechnology and Bioengineering presents the most up-to-date research on biobased technologies. It is designed to help scientists and researchers deepen their knowledge in this critical knowledge field.

core   +1 more source