Results 41 to 50 of about 102,201 (258)

Protocol for designing and preparing gallium particles using cell membrane encapsulation for applications in melanoma cryoablation therapy

STAR Protocols, 2022
Summary: Liquid metals are increasingly applied in drug delivery, in vivo imaging, and biosensing. Herein, we describe a surface modification strategy, where cell membrane is introduced to encapsulate gallium (Ga) particles.
Xuelin Wang, Xing-Jie Liang, Jie Zhang, Junge Chen, Jing Liu   +4 more
doaj   +1 more source

The human gut microbiome across the life course

FEBS Letters, EarlyView.
Despite significant individual variation and continuous change throughout life, the human gut microbiome follows some life stage‐specific trends. This article provides a brief overview of how gut microbiome composition shifts across different phases of life. Created in BioRender. Özkurt, E. (2026) https://BioRender.com/8q4nrnc.
Alise J. Ponsero, Basak Bahcivanci, Antonietta Hayhoe, Animesh Acharjee, Ezgi Özkurt   +4 more
wiley   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Current Developments in Biotechnology and Bioengineering: Advanced Membrane Separation Processes for Sustainable Water and Wastewater Management - Case Studies and Sustainability Analysis

, 2020
Current Developments in Biotechnology and Bioengineering: Advanced Membrane Separation Processes for Sustainable Water and Wastewater Management - Case Studies and Sustainability Analysis gives an up-to-date review and research developments of MBR ...
Mannina G., Larroche C., Ngo H.H., Pandey A., Ng H.Y.   +4 more
core   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Current Developments in Biotechnology and Bioengineering : Emerging Organic Micro-pollutants

, 2020
International audienceCurrent Developments in Biotechnology and Bioengineering: Emerging Organic Micropollutants summarizes the current knowledge of emerging organic micropollutants in wastewater and the possibilities of their removal/elimination.
Tiagi, Rajeshwar, Dayal, Larroche, Christian, Varjani, Sunita, Pandey, Ashok, Ngo, Huu, Hao   +4 more
core   +1 more source

Chromosomal integration of complex DNA constructs using CRAGE and CRAGE-Duet systems

STAR Protocols, 2022
Summary: Our recent development of the CRAGE (chassis-independent recombinase-assisted genome engineering) system enables single-step integration of large, complex DNA constructs directly into bacteria genomes across multiple phyla.
Zhiying Zhao, Jan-Fang Cheng, Yasuo Yoshikuni   +2 more
doaj   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Protocol to generate PDMS topographical patterns for hiPSC-derived or rat primary neuronal cultures

STAR Protocols
Summary: Soft lithography is a promising technique to fabricate tailored substrates on polydimethylsiloxane (PDMS) casts. Here, we present a protocol to generate PDMS topographical patterns for neuronal networks in vitro.
Anna-Christina Haeb, Mireia Olives-Verger, Jordi Soriano   +2 more
doaj   +1 more source

Genome editing in Penicillium funiculosum using in vitro assembled CRISPR-Cas9 ribonucleoprotein complexes

STAR Protocols, 2022
Summary: The plasmid-free CRISPR-Cas9-based genome editing in fungi is a precise and time-saving approach. Here, we present a detailed protocol for genetic manipulation in Penicillium funiculosum, which includes design and synthesis of sgRNA, high ...
Tulika Sinha, Syed Shams Yazdani
doaj   +1 more source