Results 71 to 80 of about 1,131 (245)

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Genome-wide Association Study Identifies New Loci for Resistance to Leptosphaeria maculans in Canola

Frontiers in Plant Science, 2016
Blackleg, caused by Leptosphaeria maculans, is a significant disease which affects the sustainable production of canola. This study reports a genome-wide association study based on 18,804 polymorphic SNPs to identify loci associated with qualitative and ...
Harsh Raman, Rosy Raman, Neil Coombes, Jie Song, Simon Diffey, Andrzej Kilian, Kurt Lindbeck, Denise Maria BARBULESCU, Jacqueline Batley, Dave Edwards, Philip A. Salisbury, Steve Marcroft   +11 more
doaj   +1 more source

Functional comparison of EncB and EncC cargo proteins in iron storage within the Myxococcus xanthus encapsulin

FEBS Letters, EarlyView.
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell, Thomas Walther, Egbert Hoiczyk   +2 more
wiley   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Single R gene introgression lines for accurate dissection of the Brassica - Leptosphaeria pathosystem

Frontiers in Plant Science, 2016
Seven blackleg resistance (R) genes (Rlm1, Rlm2, Rlm3, Rlm4, LepR1, LepR2 & LepR3) were each introgressed into a common susceptible B. napus doubled-haploid (DH) line through reciprocal back-crossing, producing single-R gene introgression lines (ILs) for
Nicholas Larkan, Nicholas Larkan, Fengqun Yu, Derek Lydiate, S. Roger Rimmer, M. Hossein Borhan   +5 more
doaj   +1 more source

From junk to function — How weak selection in eukaryotes builds new parts and drives genomic complexity

FEBS Letters, EarlyView.
How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley   +1 more source

Metabolomic Analysis of Rlm1-Based Blackleg Resistance

To elucidate the biochemical mechanisms underlying Rlm1-mediated blackleg resistance, we performed a metabolomic analysis comparing a susceptible DH line of Topas and its isogenic Rlm1-carrying resistant counterpart. Our results revealed significant differences in metabolite profiles post-inoculation. Rlm1-mediated resistance was characterized by early
Xiaohan Zhu, Peng Gao, Shuang Zhao, Xian Luo, Liang Li, Gary Peng   +5 more
openaire   +1 more source

Effect of Elevated CO₂ Concentration on the Disease Severity of Compatible and Incompatible Interactions of Brassica napus–Leptosphaeria maculans Pathosystem

Plants, 2019
Global warming by increased atmospheric CO2 concentration has been widely accepted. Yet, there has not been any consistent conclusion on the doubled CO2 concentration that in the future will affect plant disease incidence and severity.
Zhongwei Zou, Fei Liu, Changqin Chen, W. G. Dilantha Fernando   +3 more
doaj   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Potato Homologs of Arabidopsis thaliana Genes Functional in Defense Signaling—Identification, Genetic Mapping, and Molecular Cloning

Molecular Plant-Microbe Interactions, 2005
Defense against pests and pathogens is a fundamental process controlled by similar molecular mechanisms in all flowering plants. Using Arabidopsis thaliana as a model, steps of the signal transduction pathways that link pathogen recognition to defense ...
Karolina M. Pajerowska, Jane E. Parker, Christiane Gebhardt   +2 more
doaj   +1 more source