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WIREs Developmental Biology, 2016
The blastocyst is a mammalian invention that carries the embryo from cleavage to gastrulation. For such a simple structure, it exhibits remarkable diversity in its mode of formation, morphology, longevity, and intimacy with the uterine endometrium. This review explores this diversity in the light of the evolution of viviparity, comparing the three main
Stephen R, Frankenberg +3 more
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The blastocyst is a mammalian invention that carries the embryo from cleavage to gastrulation. For such a simple structure, it exhibits remarkable diversity in its mode of formation, morphology, longevity, and intimacy with the uterine endometrium. This review explores this diversity in the light of the evolution of viviparity, comparing the three main
Stephen R, Frankenberg +3 more
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Blastocyst Microinjection Automation
IEEE Transactions on Information Technology in Biomedicine, 2009Blastocyst microinjections are routinely involved in the process of creating genetically modified mice for biomedical research, but their efficiency is highly dependent on the skills of the operators. As a consequence, much time and resources are required for training microinjection personnel.
Leonardo S, Mattos +3 more
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2005
Experimental models of blastocyst development based on in vitro culture have played a prominent role in advancing our understanding of peri-implantation development, a process that is relatively inaccessible in vivo. Blastocyst culture provides a robust approach for examining embryonic interactions with the microenvironment under highly controlled ...
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Experimental models of blastocyst development based on in vitro culture have played a prominent role in advancing our understanding of peri-implantation development, a process that is relatively inaccessible in vivo. Blastocyst culture provides a robust approach for examining embryonic interactions with the microenvironment under highly controlled ...
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Parthenogenetic Mouse Blastocysts
Nature, 1970A READY supply of parthenogenetic mammalian embryos would be useful. Parthenogenones which develop from unfertilized eggs may be haploid, diploid or polyploid, depending on whether the meiotic divisions and first cleavage division occur1. Haploid cells can be used for studying cellular genetics and embryology.
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Pig blastocyst–uterine interactions
Differentiation, 2014The litter-bearing pig is an invaluable model for research in reproductive biology. Spherical pig blastocysts on Day 10 of pregnancy undergo rapid morphological changes to tubular and then filamentous forms by Day 12 and a filamentous conceptus of almost 1m in length by Day 16 of pregnancy.
Fuller W, Bazer, Gregory A, Johnson
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1997
Abstract Generally, a microscope with up to 300−400x magnification, two micromanipulators, two micrometer syringes, tubings, needle holders and injection chambers are needed for blastocyst injections. For the collection and handling of blastocysts a stereomicroscope (-50x magnification) is needed and a 37°C incubator (preferably with CO2
Raul M Torres, Ralf Kühn
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Abstract Generally, a microscope with up to 300−400x magnification, two micromanipulators, two micrometer syringes, tubings, needle holders and injection chambers are needed for blastocyst injections. For the collection and handling of blastocysts a stereomicroscope (-50x magnification) is needed and a 37°C incubator (preferably with CO2
Raul M Torres, Ralf Kühn
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1997
Abstract After a days work of injecting blastocysts you still must transfer these embryos to foster mothers. Initially, this can be a source of anxiety but with time can also become quite enjoyable.
Raul M Torres, Ralf Kühn
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Abstract After a days work of injecting blastocysts you still must transfer these embryos to foster mothers. Initially, this can be a source of anxiety but with time can also become quite enjoyable.
Raul M Torres, Ralf Kühn
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Mechanics of blastocyst morphogenesis
Biology of the Cell, 2017During pre‐implantation development, the mammalian zygote transforms into the blastocyst, the structure that will implant the embryo in the maternal uterus. Consisting of a squamous epithelium enveloping a fluid‐filled cavity and the inner cell mass, the blastocyst is sculpted by a succession of morphogenetic events.
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