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Mechanics of blastocyst morphogenesis

Biology of the Cell, 2017
During pre‐implantation development, the mammalian zygote transforms into the blastocyst, the structure that will implant the embryo in the maternal uterus. Consisting of a squamous epithelium enveloping a fluid‐filled cavity and the inner cell mass, the blastocyst is sculpted by a succession of morphogenetic events.
J. Maître
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Blastocyst metabolism

Reproduction, Fertility and Development, 2015
The mammalian blastocyst exhibits an idiosyncratic metabolism, reflecting its unique physiology and its ability to undergo implantation. Glucose is the primary nutrient of the blastocyst, and is metabolised both oxidatively and through aerobic glycolysis.
David K, Gardner, Alexandra J, Harvey
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Blastocyst freezing

European Journal of Obstetrics & Gynecology and Reproductive Biology, 2004
Blastocyst are preimplantation embryos that have successfully passed the critical step of genomic activation and have so a high developmental potential, thus allowing to reduce the number of embryos transferred. Blastocyst culture and freezing have really started in IVF programs in the early nineties, when it was possible to obtain good blastocysts ...
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Blastocyst Microinjection Automation

IEEE Transactions on Information Technology in Biomedicine, 2009
Blastocyst microinjections are routinely involved in the process of creating genetically modified mice for biomedical research, but their efficiency is highly dependent on the skills of the operators. As a consequence, much time and resources are required for training microinjection personnel.
Leonardo S, Mattos   +3 more
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Blastocyst Culture

2005
Experimental models of blastocyst development based on in vitro culture have played a prominent role in advancing our understanding of peri-implantation development, a process that is relatively inaccessible in vivo. Blastocyst culture provides a robust approach for examining embryonic interactions with the microenvironment under highly controlled ...
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Relationship Between Morphology, Euploidy and Implantation Potential of Cleavage and Blastocyst Stage Embryos

Journal of Human Reproductive Sciences, 2017
Aim: The aim of this study was to investigate the relationship between the morphology, euploidy and implantation rate of cleavage stage and blastocyst stage embryos. Setting: Institution-based, tertiary care in-vitro fertilization centre.
G. Majumdar   +3 more
semanticscholar   +1 more source

Parthenogenetic Mouse Blastocysts

Nature, 1970
A READY supply of parthenogenetic mammalian embryos would be useful. Parthenogenones which develop from unfertilized eggs may be haploid, diploid or polyploid, depending on whether the meiotic divisions and first cleavage division occur1. Haploid cells can be used for studying cellular genetics and embryology.
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Pig blastocyst–uterine interactions

Differentiation, 2014
The litter-bearing pig is an invaluable model for research in reproductive biology. Spherical pig blastocysts on Day 10 of pregnancy undergo rapid morphological changes to tubular and then filamentous forms by Day 12 and a filamentous conceptus of almost 1m in length by Day 16 of pregnancy.
Fuller W, Bazer, Gregory A, Johnson
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Blastocyst injedion

1997
Abstract Generally, a microscope with up to 300−400x magnification, two micromanipulators, two micrometer syringes, tubings, needle holders and injection chambers are needed for blastocyst injections. For the collection and handling of blastocysts a stereomicroscope (-50x magnification) is needed and a 37°C incubator (preferably with CO2
Raul M Torres, Ralf Kühn
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