Results 81 to 90 of about 133,185 (340)

Defining the three cell lineages of the human blastocyst by single-cell RNA-seq

Mechanisms of Development, 2015
Here, we provide fundamental insights into early human development by single-cell RNA-sequencing of human and mouse preimplantation embryos. We elucidate conserved transcriptional programs along with those that are human specific.
Paul Blakeley, N. Fogarty, I. del Valle, Sissy E. Wamaitha, T. X. Hu, K. Elder, Phil Snell, Leila Christie, P. Robson, K. Niakan   +9 more
semanticscholar   +1 more source

Genetic Deconvolution of Embryonic and Maternal Cell‐Free DNA in Spent Culture Medium of Human Preimplantation Embryo Through Deep Learning

Advanced Science, EarlyView.
DECENT is a deep learning method that enhances noninvasive preimplantation genetic testing by accurately reconstructing embryonic copy number variations (CNVs) from cell‐free DNA in spent embryo culture media. By mitigating maternal contamination, DECENT improves diagnostic accuracy, even with high contamination levels, offering a reliable, noninvasive
Zhenyi Zhang, Jie Qiao, Yidong Chen, Peijie Zhou   +3 more
wiley   +1 more source

Expression of connexins in human preimplantation embryos in vitro [PDF]

, 2004
Intercellular communication via gap junctions is required to coordinate developmental processes in the mammalian embryo. We have investigated if the connexin (Cx) isoforms known to form gap junctions in rodent preimplantation embryos are also expressed ...
Bloor, D.J., Kibschull, M., Kimber, S.J., Leese, H.J., Traub, O., Wilson, Y., Winterhager, E.   +6 more
core   +4 more sources

Cryopreservation of equine oocytes: looking into the crystal ball [PDF]

, 2020
In vitro embryo production has evolved rapidly in the horse over the past decade, but blastocyst rates from vitrified equine oocytes remain quite poor and further research is needed to warrant application.
Angel Velez, Daniel, De Coster, Tine, Smits, Katrien, Van Soom, Ann, Woelders, Henri   +4 more
core   +2 more sources

The Nuclear Localization of ACLY Guards Early Embryo Development Through Recruiting P300 and HAT1 to Promote Histone Acetylation and Transcription

Advanced Science, EarlyView.
ACLY is vital for early embryo development. IGF‐1 activates AKT to phosphorylate ACLY, driving its nuclear localization and recruitment of HATs (P300/HAT1), boosting acetyl‐CoA production and histone acetylation for transcriptional activation. Conversely, ACLY deficiency (via knockdown, knockout, or AKT inhibition) reduces nuclear acetyl‐CoA, disrupts ...
Yerong Ma, Yingyi Zhang, Weijie Yang, Xiaomei Tong, Siya Liu, Yan Zhou, Mengjia Qiu, Huifang Jiang, Zhanhong Hu, Peipei Ren, Yan Rong, Mengru Lai, Jiamin Jin, Fei Huang, Liujian Ouyang, Feng Zhou, Heng‐Yu Fan, Yin‐Li Zhang, Songying Zhang   +18 more
wiley   +1 more source

Mitochondrial reactive oxygen species regulate mitochondrial biogenesis in porcine embryos

The Journal of Reproduction and Development, 2021
The number of mitochondria in blastocysts is a potential marker of embryo quality. However, the molecular mechanisms governing the mitochondrial number in embryos are unclear.
Mio KAGEYAMA, Jun ITO, Koumei SHIRASUNA, Takehito KUWAYAMA, Hisataka IWATA   +4 more
doaj   +1 more source

TFAP2C regulates transcription in human naive pluripotency by opening enhancers. [PDF]

, 2018
Naive and primed pluripotent human embryonic stem cells bear transcriptional similarity to pre- and post-implantation epiblast and thus constitute a developmental model for understanding the pluripotent stages in human embryo development. To identify new
Chen, Di, Clark, Amander T, Ho, Jamie, Hunt, Timothy J, Jacobsen, Steven E, Kim, Rachel, Liu, Wanlu, Liu, Xiaodong, Lukianchikov, Anastasia, Pastor, William A, Polo, Jose M   +10 more
core   +2 more sources

Asynchronous fate decisions by single cells collectively ensure consistent lineage composition in the mouse blastocyst

Nature Communications, 2016
Intercellular communication is essential to coordinate the behaviour of individual cells during organismal development. The preimplantation mammalian embryo is a paradigm of tissue self-organization and regulative development; however, the cellular basis
Néstor Saiz, Kiah M. Williams, V. Seshan, A. Hadjantonakis   +3 more
semanticscholar   +1 more source

Enhanced Activities of OCT4 and SOX2 Promote Epigenetic Reprogramming by Shortening G1 Phase

Advanced Science, EarlyView.
Fusing the VP16 domain to OCT4 and SOX2 (OvSvK) enhances iPSC generation by activating downstream targets, including those regulating the cell cycle. This accelerates reprogramming by shortening the G1 phase and reducing H3K27me3 levels. Modulating Ccnd1, Cdkn2a, and Ccne1 improves efficiency, linking cell cycle to epigenetic remodeling.
Lin Guo, Jiechun Lin, Qiwen Ren, Hao Sun, Yanhua Wu, Haofei Ge, Xiaolan Wu, Lihui Lin, Lining Liang, Changpeng Li, He Liu, Yuangbang Mai, Shilong Chu, Jiadong Liu, Jing Liu, Jiekai Chen, Duanqing Pei, Hui Zheng   +17 more
wiley   +1 more source

Pluripotent Stem Cells as a Model for Human Embryogenesis

Cells, 2023
Pluripotent stem cells (PSCs; embryonic stem cells and induced pluripotent stem cells) can recapitulate critical aspects of the early stages of embryonic development; therefore, they became a powerful tool for the in vitro study of molecular mechanisms ...
Daniela Ávila-González, Mikel Ángel Gidi-Grenat, Guadalupe García-López, Alejandro Martínez-Juárez, Anayansi Molina-Hernández, Wendy Portillo, Néstor Emmanuel Díaz-Martínez, Néstor Fabián Díaz   +7 more
doaj   +1 more source