Results 1 to 10 of about 548,148 (264)

Comprehensive Optimization of Western Blotting

open access: yesGels, 2023
Western blotting is one of the most extensively used techniques in the biomedical field. However, it is criticized by many researchers due to its considerable time consumption, multiple steps, and low method results.
Dishiwen Liu   +3 more
doaj   +3 more sources

Frequency of Toxocara Antibodies in Patients Clinically Suspected to Ocular Toxocariasis, Northeast of Iran

open access: yesIranian Journal of Parasitology, 2021
Background: Human toxocariasis is a neglected parasitic disease in most countries including Iran. Among different clinical forms of toxocariasis, ocular toxocariasis (OT) is an important disease resulting in severe vision loss.
Abdolmajid Fata   +6 more
doaj   +1 more source

Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS). [PDF]

open access: yesPLoS ONE, 2015
Western blotting is a commonly used technique in biological research. A major problem with Western blotting is not the method itself, but the use of poor quality antibodies as well as the use of different experimental conditions that affect the linearity
Jennifer E Gilda   +5 more
doaj   +1 more source

Standardization and validation of a western blot for the diagnosis of human immunodeficiency virus

open access: yesRevista de la Facultad de Medicina Humana, 2021
Objectives: To standardize and validate a western blot test for the diagnosis of human immunodeficiency virus. Methods: A prospective observational study was carried out during 2017 and 2018.
Eduardo F. Miranda Ulloa   +5 more
doaj   +1 more source

Aanalysis of Western blot band patterns in HIV-1 antibody positive cases

open access: yesPifu-xingbing zhenliaoxue zazhi, 2022
Objective To analyze the epidemiological characteristics and Western blot (WB) band patterns of HIV antibody in individuals with HIV-1 antibody positive in Jiangmen city, in order to provide scientific basis for the prevention and treatment of AIDS ...
Donghe ZHANG   +3 more
doaj   +1 more source

Kinase activity-tagged western blotting assay

open access: yesBioTechniques, 2020
Determining cellular activities of protein kinases is a fundamental step for characterizing pathophysiological cell signaling pathways. Here, we optimized a nonradioactive method that detects protein kinases in tissues or cells after separation by SDS ...
Masumi Eto   +3 more
doaj   +1 more source

Comparison of Automated and Traditional Western Blotting Methods

open access: yesMethods and Protocols, 2023
Traditional Western blotting is one of the most used analytical techniques in biological research. However, it can be time-consuming and suffer from a lack of reproducibility. Consequently, devices with different degrees of automation have been developed.
Aino Sormunen   +5 more
doaj   +1 more source

Western blotting of native proteins from agarose gels

open access: yesBioTechniques, 2022
We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid.
Chiaki Sakuma   +10 more
doaj   +1 more source

The guideline for western blotting assay

open access: yesFood Frontiers, 2022
Western blotting is a technique for detecting protein developed based on protein electrophoresis separation and antigen‐antibody detection. It combines the high resolution of SDS polyacrylamide gel electrophoresis with the specificity of antigen‐antibody
Fang Wang   +5 more
doaj   +1 more source

Regulation of Expression of CXC Chemokines in Cultured Hepatocytes [PDF]

open access: yesIranian Journal of Public Health, 2005
Chemokines are low molecular weight proteins (8-17 kDa). They are classified in four distinct groups as CXC, CC, CX3C and C. Depending on the presence or absence of a motif called ELR (Arg-Leu-Glu) before the first cysteine residue in their structure ...
GhH Hassanshahiraviz   +1 more
doaj   +1 more source

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