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Comprehensive Optimization of Western Blotting
Western blotting is one of the most extensively used techniques in the biomedical field. However, it is criticized by many researchers due to its considerable time consumption, multiple steps, and low method results.
Dishiwen Liu+3 more
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Background: Human toxocariasis is a neglected parasitic disease in most countries including Iran. Among different clinical forms of toxocariasis, ocular toxocariasis (OT) is an important disease resulting in severe vision loss.
Abdolmajid Fata+6 more
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Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS). [PDF]
Western blotting is a commonly used technique in biological research. A major problem with Western blotting is not the method itself, but the use of poor quality antibodies as well as the use of different experimental conditions that affect the linearity
Jennifer E Gilda+5 more
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Standardization and validation of a western blot for the diagnosis of human immunodeficiency virus
Objectives: To standardize and validate a western blot test for the diagnosis of human immunodeficiency virus. Methods: A prospective observational study was carried out during 2017 and 2018.
Eduardo F. Miranda Ulloa+5 more
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Aanalysis of Western blot band patterns in HIV-1 antibody positive cases
Objective To analyze the epidemiological characteristics and Western blot (WB) band patterns of HIV antibody in individuals with HIV-1 antibody positive in Jiangmen city, in order to provide scientific basis for the prevention and treatment of AIDS ...
Donghe ZHANG+3 more
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Kinase activity-tagged western blotting assay
Determining cellular activities of protein kinases is a fundamental step for characterizing pathophysiological cell signaling pathways. Here, we optimized a nonradioactive method that detects protein kinases in tissues or cells after separation by SDS ...
Masumi Eto+3 more
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Comparison of Automated and Traditional Western Blotting Methods
Traditional Western blotting is one of the most used analytical techniques in biological research. However, it can be time-consuming and suffer from a lack of reproducibility. Consequently, devices with different degrees of automation have been developed.
Aino Sormunen+5 more
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Western blotting of native proteins from agarose gels
We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid.
Chiaki Sakuma+10 more
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The guideline for western blotting assay
Western blotting is a technique for detecting protein developed based on protein electrophoresis separation and antigen‐antibody detection. It combines the high resolution of SDS polyacrylamide gel electrophoresis with the specificity of antigen‐antibody
Fang Wang+5 more
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Regulation of Expression of CXC Chemokines in Cultured Hepatocytes [PDF]
Chemokines are low molecular weight proteins (8-17 kDa). They are classified in four distinct groups as CXC, CC, CX3C and C. Depending on the presence or absence of a motif called ELR (Arg-Leu-Glu) before the first cysteine residue in their structure ...
GhH Hassanshahiraviz+1 more
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