Results 11 to 20 of about 2,923,932 (298)
Western blotting (immunoblotting): history, theory, uses, protocol and problems
BioTechniques, 2023 Western blotting (immunoblotting) is a powerful and commonly used technique that is capable of detecting or semiquantifying an individual protein from complex mixtures of proteins extracted from cells or tissues.
Rasheed Sule +2 more
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Regulation of Expression of CXC Chemokines in Cultured Hepatocytes [PDF]
Iranian Journal of Public Health, 2005 Chemokines are low molecular weight proteins (8-17 kDa). They are classified in four distinct groups as CXC, CC, CX3C and C. Depending on the presence or absence of a motif called ELR (Arg-Leu-Glu) before the first cysteine residue in their structure ...
GhH Hassanshahiraviz, AJ Dickson
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Antibody Purification from Western Blotting
Bio-Protocol, 2012 This protocol describes a method of purifying antibodies from sera with denatured antigens immobilized on western blot membranes. Advantages include (1) fast and easy; (2) purification of antibody with antigen in denatured form allows high yield in case ...
Lin Fang
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Microfluidic Western blotting [PDF]
Proceedings of the National Academy of Sciences, 2012 Rapid, quantitative Western blotting is a long-sought bioanalytical goal in the life sciences. To this end, we describe a Western blotting assay conducted in a single glass microchannel under purely electronic control. The μWestern blot is comprised of multiple steps: sample enrichment, protein sizing, protein immobilization (blotting), and in situ ...
Alex J, Hughes, Amy E, Herr
openaire +2 more sources
Journal of Advances in VetBio Science and Techniques, 2021 Western Blot tekniği, herhangi bir örnekten istenen tek bir proteini özgün bir şekilde saptamak için moleküler biyolojide kullanılan eski ve güvenilir bir yöntemdir. Proteinlerin uyaranlara karşı verdiği yanıtların araştırılmasında, protein ifadesinin var-yok ya da artmış-azalmış olarak değerlendirilmesinde, hücre spesifik protein izoformlarının ...
Hatice Pelin ASLIM, Oya BULUT
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Detection of Zebrafish Retinal Proteins by Infrared Western Blotting
Bio-Protocol, 2023 The zebrafish retina is a canonical vertebrate retina. Since the past few years, with the continually growing genetic toolbox and imaging techniques, zebrafish plays a crucial role in retinal research.
Jingjing Zang, Stephan Neuhauss
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Antibodies used for Western blotting.
, 2021 Antibodies used for Western blotting.
Bhawna Yadav (172612) +6 more
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Western Blotting for Staphylococcus aureus AgrA
Bio-Protocol, 2014 Staphylococcus aureus has a quorum sensing system to regulate the expression of various virulence factors, which is exerted by the agr locus that encodes agrBDCA and a regulatory RNA called RNAIII.
Chikara Kaito, Kazuhisa Sekimizu
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Virus Overlay Assay (Far-Western blotting)
Bio-Protocol, 2013 Virus overlay assay is a method to detect protein-protein interaction in vitro. We performed the virus overlay assay to identify the receptor proteins interacting with the infectious spleen and kidney necrosis virus (ISKNV).
Kun-Tong Jia +3 more
doaj +1 more source