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Polyacrylamide Gel Electrophoresis [PDF]
Cold Spring Harbor Protocols, 2020 Cross-linked chains of polyacrylamide can be used as electrically neutral gels to separate double-stranded DNA fragments according to size and single-stranded DNAs according to size and conformation. Polyacrylamide gels have the following three major advantages over agarose gels: (1) Their resolving power is so great that they can separate molecules of
Michael R, Green, Joseph, Sambrook
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Denaturing Polyacrylamide Gel Electrophoresis [PDF]
Current Protocols in Nucleic Acid Chemistry, 1994 AbstractThin polyacrylamide gels that contain a high concentration of urea as a denaturant are capable of resolving short (<500 nucleotides) single‐stranded fragments of DNA or RNA that differ in length by as little as one nucleotide. Such gels are uniquely suited for nucleic acid sequence analysis, which is required, for instance, for all ...
Albright, Lisa M., Slatko, Barton E.
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The electrophoresis of transferrins in urea/polyacrylamide gels [PDF]
Biochemical Journal, 1980 The denaturation of transferrin by urea has been studied by (a) electrophoresis in polyacrylamide gels incorporating a urea gradient, (b) measurements of the loss of iron-binding capacity and (c) u.v. difference spectrometry. In human serum transferrin and hen ovotransferrin the N-terminal and C-terminal domains of the iron-free protein were found to ...
Evans, RW, Williams, J
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A Viscosity Model of Polyacrylamide Gel Electrophoresis
Zeitschrift für Naturforschung C, 1979 In current theories of polyacrylamide gel electrophoresis, the idea prevails that molecular sieving relies on different accessibility of volume fractions and of cross-sectional area fractions (denoted “pores”) to different-sized ions due to the effect of “geometric exclusion”.
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Polyacrylamide Gel Electrophoresis
Drug Delivery System, 2023 Yoshimasa Kawaguchi, Yu Mikame
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Discontinuous Electrophoresis on Polyacrylamide Gel Slab
Clinical Chemistry, 1969 Abstract An apparatus for discontinuous electrophoresis on a slab of polyacrylamide gel is described. It affords resolution of serum protein as distinct as that with electrophoresis on a column of gel, but with considerable simplification in technic.
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Polyacrylamide Gel Electrophoresis
Science, 1971Polyacrylamide gel electrophoresis (PAGE) provides a versatile, gentle, high resolution method for fractionation and physical-chemical characterization of molecules on the basis of size, conformation, and net charge. The polymerization reaction can be rigorously controlled to provide uniform gels of reproducible, measurable pore size over ...
A, Chrambach, D, Rodbard
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ELECTROPHORESIS OF HISTONES ON POLYACRYLAMIDE GELS
Canadian Journal of Biochemistry and Physiology, 1963Histories extracted from various organs of the rat have been fractionated by electrophoresis on Polyacrylamide gels. The most convenient reagents to form the gel were selected and the effects of varying concentrations of these reagents on the resolution of histones were investigated.
A, DRIEDGER, L D, JOHNSON, A M, MARKO
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C3 Polymorphism in Polyacrylamide‐Gel Electrophoresis
Vox Sanguinis, 1976Abstract. C3 polymorphism in polyacrylamide‐gel electrophoresis was identified by crossed immunoelectrophoresis using anti‐C3/C3c‐serum. Through ageing, treatment of sera with cobra venom factor, endotoxin or with neuraminidase, polymorphic bands were seen also in a conversion product and antigenically attributed to C3c.
J, Schwamborn, H, Freis, G, Mauff
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