Results 41 to 50 of about 626,288 (358)

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

The electrophoresis of transferrins in urea/polyacrylamide gels [PDF]

Biochemical Journal, 1980
The denaturation of transferrin by urea has been studied by (a) electrophoresis in polyacrylamide gels incorporating a urea gradient, (b) measurements of the loss of iron-binding capacity and (c) u.v. difference spectrometry. In human serum transferrin and hen ovotransferrin the N-terminal and C-terminal domains of the iron-free protein were found to ...
Evans, RW, Williams, J
openaire   +3 more sources

Analysis of the effects of BrdU on DLKP human lung cancer cells by two-dimensional difference gel electrophoresis and mass spectrometry [PDF]

, 2007
Bromodeoxyuridine (BrdU) is a thymidiie analogue that incorporates into DNA of dividing cells during the S-phase of the cell cycle. Previous work in laboratories reported that treatment with lOyM BrdU in the human lung carcinoma cell line (DLKP) resulted
Henry, Michael
core  

Polyacrylamide Gel with Switchable Trypsin Activity for Analysis of Proteins

, 2013
Trypsin was immobilized on a variety of materials to improve digestion efficiency. However, because the immobilized trypsin will digest proteins during electrophoresis, direct immobilization of active trypsin in polyacrylamide gel will compromise the ...
Qin, Hongqiang, 叶明亮, Hu, Zhengyan, Zou, Hanfa, 邹汉法, Liu, Fangjie, Zhang, Yi, Ye, Mingliang, Wang, Chunli, Cheng, Kai   +9 more
core   +1 more source

A preparation of Alzheimer paired helical filaments that displays distinct tau proteins by polyacrylamide gel electrophoresis.

Proceedings of the National Academy of Sciences of the United States of America, 1990
Paired helical filaments (PHFs) are prominent components of Alzheimer disease (AD) neurofibrillary tangles (NFTs). Rather than isolating NFTs, we selected for PHF populations that can be extracted from AD brain homogenates.
S. G. Greenberg, P. Davies
semanticscholar   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source

Disassembly of electrophoresis in polyacrylamide gel and gel staining

, 2021
For the visualization of the separation of proteins by electrophoresis in polyacrylamide gel, once the electrophoresis is performed, the gel will be taken out of the system and the proteins stained, as explained in this video.https://media.upv.es/player/?
Yenush, Lynne, Andrés-Colás, Nuria
core  

Useful Buffer and Gel Systems for Polyacrylamide Gel Electrophoresis [PDF]

, 1971
Seven buffer and gel systems for polyacrylamide gel electrophoresis using discontinuous voltage and pH gradients (disc electrophoresis according to ORNSTEIN-UAVIS) and five systems using discontinuous voltage gradients at constant pH (according to ALLEN)
H. R. Maurer, Maurer, H. R., Allen, R. C., R. C. Allen   +3 more
core   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Detection of rotavirus genome by new silver staining method

مجله دانشگاه علوم پزشکی گرگان, 2014
Background and Objective: Rotaviruses are the members of the Reoviridae family containing double-stranded RNA (dsRNA) genome which are the main cause of gastroinentritis particularly in children less than three years.
Ahmadi E, Soleimanjahi H, Teimoori A
doaj