Results 31 to 40 of about 626,288 (358)
An Improved Method for PAGE-based Detection of Phosphorylated Protein in Yeast
Bio-Protocol, 2012 One dimensional polyacrylamide gel electrophoresis has been successfully used to detect protein phosphorylation. This method is very simple and highly reproducible.
Yuehua Wei
doaj +1 more source
Electrophoresis in polyacrylamide gel
, 2021 For the separation of proteins by electrophoresis in polyacrylamide gel, once the gel is prepared and the electrophoresis system assembled, the electrophoresis buffer will be added and the protein samples loaded to proceed with the electrophoresis, as ...
Yenush, Lynne, Andrés-Colás, Nuria
core
Journal of Biological Chemistry, 1974 The application of a method of electrophoretic protein analysis to the investigation of the total protein composition of Salmonella typhimurium is described.
G. Ames
semanticscholar +1 more source
, 2011 Efficient separation of mucins (200 kDa-2 MDa) was demonstrated using gradient SDS agarose/polyacrylamide composite gel electrophoresis (SDS-AgPAGE). Inclusion of urea (SDS-UAgPAGE) in the gels casting were shown to have no effect on the migration of ...
Packer, NH +3 more
core +2 more sources
Analytical Biochemistry, 2011 Agarose and polyacrylamide gel electrophoresis systems for the molecular mass-dependent separation of hyaluronan (HA) in the size range of approximately 5–500 kDa have been investigated.
Shardul Bhilocha +10 more
semanticscholar +1 more source
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Apolipophorin III in locusts: purification and characterization.
Journal of Lipid Research, 1988 Three molecular species of apolipophorin III were purified from adult locust hemolymph by gel filtration and ion-exchange chromatography, and named apo-III-a, apo-III-b, and apo-III-c, respectively.
H Chino, M Yazawa
doaj +1 more source
Assembly for electrophoresis in polyacrylamide gel
, 2021 For the separation of proteins by electrophoresis in polyacrylamide gel, once the gel is already prepared, the system where to perform the electrophoresis will be assembled, as explained in this video.https://media.upv.es/player/?id=65122530-8273-11eb ...
Yenush, Lynne, Andrés-Colás, Nuria
core
FEBS Letters, EarlyView.This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley +1 more source
Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE)
, 2008 QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point.
Bernd Kastenholz
core +1 more source