Results 11 to 20 of about 626,288 (358)

Polyacrylamide gel electrophoresis of soybean seed proteins

open access: yesActa Societatis Botanicorum Poloniae, 2015
Four major and 14 minor protein bands were detected when total salt soluble proteins of soybean (Glycine max cultivar Warszawska) seed were subjected to polyacrylamide gel electrophoresis under nondissociating conditions, and 16 protein bands were ...
W. Lassocińsk, J. S. Knypl
doaj   +3 more sources

Purification of Peptide Components including Melittin from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis

open access: yesJournal of Pharmacopuncture, 2006
Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Methods : Melittin and other peptide ...
Young Chon Choi   +2 more
doaj   +2 more sources

The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

open access: yesJournal of Biological Chemistry, 1969
Forty proteins with polypeptide chains of well characterized molecular weights have been studied by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate following the procedure of Shapiro, Vinuela, and Maizel (Biochem.
K. Weber, M. Osborn
semanticscholar   +1 more source

Analysis of mitochondrial respiratory chain supercomplexes using blue native polyacrylamide gel electrophoresis (BN-PAGE)

open access: yesCurrent Protocols in Mouse Biology, 2016
Mitochondria are cellular organelles that harvest energy in the form of ATP through a process termed oxidative phosphorylation (OXPHOS), which occurs via the protein complexes of the electron transport chain (ETC).
Pooja Jha, Xu Wang, J. Auwerx
semanticscholar   +1 more source

Stochastic modeling for the COMET-assay [PDF]

open access: yes, 2003
We present a stochastic model for single cell gel electrophoresis (COMET-assay) data. Essential is the use of point process structures, renewal theory and reduction to intensity histograms for further data ...
Hösel, V.   +4 more
core   +1 more source

Polyacrylamide gel electrophoresis: a versatile tool for the separation of nanoclusters

open access: yesBioTechniques, 2023
Atomically precise nanoclusters comprising 1–100 atoms have emerged as a new class of nanomaterials with intriguing size-dependent physicochemical properties. The significant changes in the properties of nanoclusters were observed in tailoring the number
Vinitha Packirisamy, Prabhu Pandurangan
doaj   +1 more source

Basics and recent advances of two dimensional- polyacrylamide gel electrophoresis

open access: yesClinical Proteomics, 2014
Gel- based proteomics is one of the most versatile methods for fractionating protein complexes. Among these methods, two dimensional- polyacrylamide gel electrophoresis (2-DE) represents a mainstay orthogonal approach, which is popularly used to ...
Sameh Magdeldin   +8 more
semanticscholar   +1 more source

A versatile polyacrylamide gel electrophoresis based sulfotransferase assay

open access: yesBMC Biotechnology, 2010
Background Sulfotransferases are a large group of enzymes that regulate the biological activity or availability of a wide spectrum of substrates through sulfation with the sulfur donor 3'-phosphoadenosine-5'-phosphosulfate (PAPS). These enzymes are known
Prather Brittany   +4 more
doaj   +1 more source

SDS-polyacrylamide gel electrophoresis of SpGrx3 WT and mutants.

open access: yes, 2023
SDS-polyacrylamide gel electrophoresis of SpGrx3 WT and mutants.
Sei-Heon Jang (2828522)   +3 more
core   +1 more source

Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE): advances and perspectives

open access: yesBioTechniques, 2008
The recent trend in science is to assay as many biological molecules as possible within a single experiment. This trend is evident in proteomics where the aim is to characterize thousands of proteins within cells, tissues, and organisms.
Haleem J. Issaq, Timothy D. Veenstra
doaj   +1 more source

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