Results 11 to 20 of about 517,672 (313)

Carbohydrate Affinity PAGE for the Study of Carbohydrate-Binding Proteins

open access: yesBioTechniques, 1998
Immobilized neoglycoconjugates covalently cross-linked into a polyacrylamide gel can be used to detect and characterize carbohydrate-binding proteins.
Phil D. Rye, Nicolai V. Bovin
doaj   +1 more source

Polyacrylamide gel electrophoresis of soybean seed proteins

open access: yesActa Societatis Botanicorum Poloniae, 2015
Four major and 14 minor protein bands were detected when total salt soluble proteins of soybean (Glycine max cultivar Warszawska) seed were subjected to polyacrylamide gel electrophoresis under nondissociating conditions, and 16 protein bands were ...
W. Lassocińsk, J. S. Knypl
doaj   +1 more source

Purification of Peptide Components including Melittin from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis

open access: yesJournal of Pharmacopuncture, 2006
Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Methods : Melittin and other peptide ...
Young Chon Choi   +2 more
doaj   +1 more source

SDS Agarose Gels for Analysis of Proteins

open access: yesBioTechniques, 1998
A new agarose-based protein electrophoresis gel system is described. The system consists of a highly resolving agarose, MetaPhor® XR (FMC BioProducts, Rockland, ME, USA) dissolved in urea and TBE buffer and a stacking gel composed of a high gel strength ...
Minjie Wu, Noriko Kusukawa
doaj   +1 more source

Power and limitations of electrophoretic separations in proteomics strategies [PDF]

open access: yes, 2009
Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry.
Aebersold   +225 more
core   +4 more sources

Polyacrylamide gel electrophoresis: a versatile tool for the separation of nanoclusters

open access: yesBioTechniques, 2023
Atomically precise nanoclusters comprising 1–100 atoms have emerged as a new class of nanomaterials with intriguing size-dependent physicochemical properties. The significant changes in the properties of nanoclusters were observed in tailoring the number
Vinitha Packirisamy, Prabhu Pandurangan
doaj   +1 more source

Sweet silver: A formaldehyde-free silver staining using aldoses as developing agents, with enhanced compatibility with mass spectrometry [PDF]

open access: yes, 2008
Protein detection methods after electrophoresis have to be sensitive, homogeneous, and not to impair downstream analysis of proteins by MS. Speed, low cost, and user friendliness are also favored features. Silver staining combines many of these features,
Berggren   +26 more
core   +4 more sources

A versatile polyacrylamide gel electrophoresis based sulfotransferase assay

open access: yesBMC Biotechnology, 2010
Background Sulfotransferases are a large group of enzymes that regulate the biological activity or availability of a wide spectrum of substrates through sulfation with the sulfur donor 3'-phosphoadenosine-5'-phosphosulfate (PAPS). These enzymes are known
Prather Brittany   +4 more
doaj   +1 more source

Membrane proteins and proteomics: Love is possible, but so difficult [PDF]

open access: yes, 2009
Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully balanced hydrophilic and lipophilic environment, which optimum varies with ...
Adessi   +103 more
core   +5 more sources

Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE): advances and perspectives

open access: yesBioTechniques, 2008
The recent trend in science is to assay as many biological molecules as possible within a single experiment. This trend is evident in proteomics where the aim is to characterize thousands of proteins within cells, tissues, and organisms.
Haleem J. Issaq, Timothy D. Veenstra
doaj   +1 more source

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