Results 1 to 10 of about 849,075 (295)
Single-cell western blotting. [PDF]
Nature Reviews Molecular Cell Biology, 2014 To measure cell-to-cell variation in protein-mediated functions, we developed an approach to conduct ∼10(3) concurrent single-cell western blots (scWesterns) in ∼4 h.
Herr, Amy E +5 more
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The guideline for western blotting assay
Food Frontiers, 2022 Western blotting is a technique for detecting protein developed based on protein electrophoresis separation and antigen‐antibody detection. It combines the high resolution of SDS polyacrylamide gel electrophoresis with the specificity of antigen‐antibody
Fang Wang +5 more
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Comparison of Automated and Traditional Western Blotting Methods
Methods and Protocols, 2023 Traditional Western blotting is one of the most used analytical techniques in biological research. However, it can be time-consuming and suffer from a lack of reproducibility. Consequently, devices with different degrees of automation have been developed.
Aino Sormunen +5 more
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Microfluidic Western blotting [PDF]
Proceedings of the National Academy of Sciences, 2012 Rapid, quantitative Western blotting is a long-sought bioanalytical goal in the life sciences. To this end, we describe a Western blotting assay conducted in a single glass microchannel under purely electronic control. The μWestern blot is comprised of multiple steps: sample enrichment, protein sizing, protein immobilization (blotting), and in situ ...
Alex J, Hughes, Amy E, Herr
openaire +2 more sources
Western blotting of native proteins from agarose gels
BioTechniques, 2022 We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid.
Chiaki Sakuma +10 more
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Measuring protein levels in planarians using western blotting
STAR Protocols, 2021 Summary: In the planarian field, two techniques are mostly used for protein detection: immunohistochemistry (IHC) and western blotting. While IHC is great for visualizing the spatial distribution of proteins in whole organisms, it has limitations in ...
Benjamin Ziman, Néstor J. Oviedo
doaj +1 more source
Regulation of Expression of CXC Chemokines in Cultured Hepatocytes [PDF]
Iranian Journal of Public Health, 2005 Chemokines are low molecular weight proteins (8-17 kDa). They are classified in four distinct groups as CXC, CC, CX3C and C. Depending on the presence or absence of a motif called ELR (Arg-Leu-Glu) before the first cysteine residue in their structure ...
GhH Hassanshahiraviz, AJ Dickson
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Comparisons of ELISA and Western blot assays for detection of autophagy flux [PDF]
, 2017 We analyzed autophagy/mitophagy flux in vitro (C2C12 myotubes) and in vivo (mouse skeletal muscle) following the treatments of autophagy inducers (starvation, rapamycin) and a mitophagy inducer (carbonyl cyanide m-chlorophenylhydrazone, CCCP) using two ...
Choi, Yong-bok +4 more
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Antibody Purification from Western Blotting
Bio-Protocol, 2012 This protocol describes a method of purifying antibodies from sera with denatured antigens immobilized on western blot membranes. Advantages include (1) fast and easy; (2) purification of antibody with antigen in denatured form allows high yield in case ...
Lin Fang
doaj +1 more source
The protein antigens secreted in vivo by adult male Schistosoma mansoni [PDF]
, 1997 Adult schistosomes can be transferred surgically from donor C57BL/6 mice to the portal veins of naive recipients with complete success. This procedure bypasses larval development and the antibody response of the host is directed against, and can be used ...
Cutts, L., Wilson, R.A.
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