Results 1 to 10 of about 2,923,932 (298)
Comprehensive Optimization of Western Blotting
Gels, 2023 Western blotting is one of the most extensively used techniques in the biomedical field. However, it is criticized by many researchers due to its considerable time consumption, multiple steps, and low method results.
Dishiwen Liu +3 more
doaj +1 more source
Iranian Journal of Parasitology, 2021 Background: Human toxocariasis is a neglected parasitic disease in most countries including Iran. Among different clinical forms of toxocariasis, ocular toxocariasis (OT) is an important disease resulting in severe vision loss.
Abdolmajid Fata +6 more
doaj +1 more source
Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS). [PDF]
PLoS ONE, 2015 Western blotting is a commonly used technique in biological research. A major problem with Western blotting is not the method itself, but the use of poor quality antibodies as well as the use of different experimental conditions that affect the linearity
Jennifer E Gilda +5 more
doaj +1 more source
Aanalysis of Western blot band patterns in HIV-1 antibody positive cases
Pifu-xingbing zhenliaoxue zazhi, 2022 Objective To analyze the epidemiological characteristics and Western blot (WB) band patterns of HIV antibody in individuals with HIV-1 antibody positive in Jiangmen city, in order to provide scientific basis for the prevention and treatment of AIDS ...
Donghe ZHANG +3 more
doaj +1 more source
Standardization and validation of a western blot for the diagnosis of human immunodeficiency virus
Revista de la Facultad de Medicina Humana, 2021 Objectives: To standardize and validate a western blot test for the diagnosis of human immunodeficiency virus. Methods: A prospective observational study was carried out during 2017 and 2018.
Eduardo F. Miranda Ulloa +5 more
doaj +1 more source
Kinase activity-tagged western blotting assay
BioTechniques, 2020 Determining cellular activities of protein kinases is a fundamental step for characterizing pathophysiological cell signaling pathways. Here, we optimized a nonradioactive method that detects protein kinases in tissues or cells after separation by SDS ...
Masumi Eto +3 more
doaj +1 more source
Comparison of Automated and Traditional Western Blotting Methods
Methods and Protocols, 2023 Traditional Western blotting is one of the most used analytical techniques in biological research. However, it can be time-consuming and suffer from a lack of reproducibility. Consequently, devices with different degrees of automation have been developed.
Aino Sormunen +5 more
doaj +1 more source
Western blotting of native proteins from agarose gels
BioTechniques, 2022 We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid.
Chiaki Sakuma +10 more
doaj +1 more source
Measuring protein levels in planarians using western blotting
STAR Protocols, 2021 Summary: In the planarian field, two techniques are mostly used for protein detection: immunohistochemistry (IHC) and western blotting. While IHC is great for visualizing the spatial distribution of proteins in whole organisms, it has limitations in ...
Benjamin Ziman, Néstor J. Oviedo
doaj +1 more source