Results 121 to 130 of about 666,894 (260)

Reverse–normal immunopurification: An effective approach for purifying recombinant erythropoietin from its analogues in doping analysis

Journal of Sport and Health Science
Background: Recombinant erythropoietin (rEPO) is commonly used in therapy but may be abused in sports to enhance endurance. In doping analysis, rEPO can be detected in human urine or blood samples at picogram (pg) levels based on its slightly higher ...
Sen He, Die Wu, Chengshuai Niu, Xinchao Liu, Jie Zhang, Liangzhi Xie, Laurent Martin, Kaifeng Liu, Xinmiao Zhou, Lisi Zhang   +9 more
doaj   +1 more source

Determination of Diagnostic Antigens in Cattle Amphistomiasis Using Western Blotting [PDF]

Iranian Journal of Parasitology, 2009
"nBackground: Mixed infection with amphistomes seems common in native cattle of Iran. The aim of this study was to determine diagnostic antigens in cattle mixed amphistomiasis."nMethods: Specific antigens of Cotylophoron cotylophorum, Gastrothylax ...
A Halajian, A Eslami, B Meshgi, B Meshgi, A Eslami, A Halajian   +5 more
doaj  

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Antibodies used in western blotting assay.

, 2015
Antibodies used in western blotting assay.
Kaichun Wu (124044), Zhiping Yang (644241), Dongmei Fan (785349), Daiming Fan (124057), Qingchuan Zhao (252001), Yongzhan Nie (124038), Ying Wang (11406), Po Xu (785350)   +7 more
core   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Antibodies used for immunofluorescence and western blotting.

, 2013
Antibodies used for immunofluorescence and western blotting.
Martine Muckenstrum (332589), Marie-Pierre Chenard (269908), Adrien Rousseau (332587), Frederic Gambino (5652514), Fabien Alpy (269903), Nadia Messadeq (332588), Catherine Tomasetto (269910), Yann Humeau (186719), Marie Kneib (332586), Marie-Christine Rio (269912), Sébastien Blaise (332585)   +10 more
core   +1 more source

Comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of Paraná, Brazil

Brazilian Journal of Infectious Diseases
We evaluated the effectiveness of serological and parasitological methods for cutaneous leishmaniasis (CL) diagnosis in patients from the central region of Paraná state, southern Brazil.
Regiane Szargiki, Edilene Alcântara de Castro, Ennio Luz, Wolodymir Kowalthuk, Ângela Maria Machado, Vanete Thomaz-Soccol   +5 more
doaj   +1 more source

Identification of Aeromonas hydrophila infection with specific monoclonal antibodies [PDF]

Maejo International Journal of Science and Technology, 2007
Whole cell of Aeromonas hydrophila 1234 was used for immunization to produce monoclonal antibodies (MAbs). Three different groups of MAbs specific to Aeromonas were obtained.
Paisarn Sithigorngul
doaj  

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source