Results 231 to 240 of about 548,148 (264)
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2021
Every cell contains several proteins. How do we identify every protein in the cell? This question vexed many scientists until the introduction of the procedure known as protein blotting or Western blotting. Cells obtained from tissues or cells grown outside the human body in a plastic flask are first busted to release its contents.
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Every cell contains several proteins. How do we identify every protein in the cell? This question vexed many scientists until the introduction of the procedure known as protein blotting or Western blotting. Cells obtained from tissues or cells grown outside the human body in a plastic flask are first busted to release its contents.
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1986
This chapter provides an overview of the western blot analysis method, which allows the investigator to identify specific proteins resolved by SDS polyacrylamide gel electrophoresis by binding with specific antisera. Proteins resolved on an acrylamide gel are transferred to a nitrocellulose (NC) filter that is incubated with the antisera.
Mark D. Dibner +2 more
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This chapter provides an overview of the western blot analysis method, which allows the investigator to identify specific proteins resolved by SDS polyacrylamide gel electrophoresis by binding with specific antisera. Proteins resolved on an acrylamide gel are transferred to a nitrocellulose (NC) filter that is incubated with the antisera.
Mark D. Dibner +2 more
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Western Blotting Using Microfluidics
2015Together with polyacrylamide gel electrophoresis, the western blot has been an invaluable research technique in biological sciences. It continues to serve as an important diagnostic tool in medical laboratories. The procedure, however, involves multiple steps that are often time and resource intensive in addition to being of low throughput.
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Current treatment and recent progress in gastric cancer
Ca-A Cancer Journal for Clinicians, 2021Smita S Joshi, Brian D Badgwell
exaly
2000
Proteins can be separated according to their molecular sizes and charges, since these factors will determine the speed at which they will travel through a gel. The SDS-PAGE method involves the denaturation of proteins with the detergent sodium dodecyl sulfate (SDS) and the use of an electric current to pull them through a polyacrylamide gel, a process ...
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Proteins can be separated according to their molecular sizes and charges, since these factors will determine the speed at which they will travel through a gel. The SDS-PAGE method involves the denaturation of proteins with the detergent sodium dodecyl sulfate (SDS) and the use of an electric current to pull them through a polyacrylamide gel, a process ...
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Long‐term variability and trends in meteorological droughts in Western Europe (1851–2018)
International Journal of Climatology, 2021Neil Macdonald +2 more
exaly
, 2006