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Tropical gastroenterology : official journal of the Digestive Diseases Foundation, 1989 P, Sharma +3 more
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Methods, 2006
Western blotting (protein blotting or immunoblotting) is a powerful and important procedure for the immunodetection of proteins post-electrophoresis, particularly proteins that are of low abundance. Since the inception of the protocol for protein transfer from an electrophoresed gel to a membrane in 1979, protein blotting has evolved greatly.
Biji T, Kurien, R Hal, Scofield
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Western blotting (protein blotting or immunoblotting) is a powerful and important procedure for the immunodetection of proteins post-electrophoresis, particularly proteins that are of low abundance. Since the inception of the protocol for protein transfer from an electrophoresed gel to a membrane in 1979, protein blotting has evolved greatly.
Biji T, Kurien, R Hal, Scofield
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Molecular Biotechnology, 1994
The different steps involved in protein (Western) blotting and subsequent analysis of the proteins are reviewed. Electrophoretic separation of proteins, procedures of transfer to membranes, immunological and nonimmunological protein detection systems, and characterization of protein-nucleic acid and protein-protein interactions are described.
D, Egger, K, Bienz
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The different steps involved in protein (Western) blotting and subsequent analysis of the proteins are reviewed. Electrophoretic separation of proteins, procedures of transfer to membranes, immunological and nonimmunological protein detection systems, and characterization of protein-nucleic acid and protein-protein interactions are described.
D, Egger, K, Bienz
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Analytical Chemistry, 2010
We develop a novel method for Western blot based on microfluidics, incorporating the internal molecular weight marker, loading control, and antibody titration in the same protocol. Compared with the conventional method which could detect only one protein, the microfluidic Western blot could analyze at least 10 proteins simultaneously from a single ...
Wenying, Pan, Wei, Chen, Xingyu, Jiang
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We develop a novel method for Western blot based on microfluidics, incorporating the internal molecular weight marker, loading control, and antibody titration in the same protocol. Compared with the conventional method which could detect only one protein, the microfluidic Western blot could analyze at least 10 proteins simultaneously from a single ...
Wenying, Pan, Wei, Chen, Xingyu, Jiang
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2003
Western blotting is employed for the detection of proteins and other macromolecules immobilized on nitrocellulose membranes. This rapid and sensitive method enables the identification and quantification of a specific protein from cell lysates or a mixture of proteins.
N R, Madamanchi, M S, Runge
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Western blotting is employed for the detection of proteins and other macromolecules immobilized on nitrocellulose membranes. This rapid and sensitive method enables the identification and quantification of a specific protein from cell lysates or a mixture of proteins.
N R, Madamanchi, M S, Runge
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2015
Western blotting is a technique that involves the separation of proteins by gel electrophoresis, their blotting or transfer to a membrane, and selective immunodetection of an immobilized antigen. This is an important and routine method for protein analysis that depends on the specificity of antibody-antigen interaction and is useful for the qualitative
Thomas S, Hnasko, Robert M, Hnasko
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Western blotting is a technique that involves the separation of proteins by gel electrophoresis, their blotting or transfer to a membrane, and selective immunodetection of an immobilized antigen. This is an important and routine method for protein analysis that depends on the specificity of antibody-antigen interaction and is useful for the qualitative
Thomas S, Hnasko, Robert M, Hnasko
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2003
Western blotting (reviewed in 1-3; see also this vol., Chapter 24 ) refers to formation and detection of an antibody-antigen complex between an antibody and a polypeptide that is immobilized on derivatized paper.
S H, Kaufmann, J H, Shaper
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Western blotting (reviewed in 1-3; see also this vol., Chapter 24 ) refers to formation and detection of an antibody-antigen complex between an antibody and a polypeptide that is immobilized on derivatized paper.
S H, Kaufmann, J H, Shaper
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2013
Western analysis of apolipoproteins, lipoproteins, and proteins involved in lipoprotein metabolism can be challenging due to their size, hydrophobic nature, and, in some cases, low abundance. Here we describe a Western blotting method that has been used successfully for many proteins involved in lipoprotein metabolism, as well as intact LDL or HDL ...
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Western analysis of apolipoproteins, lipoproteins, and proteins involved in lipoprotein metabolism can be challenging due to their size, hydrophobic nature, and, in some cases, low abundance. Here we describe a Western blotting method that has been used successfully for many proteins involved in lipoprotein metabolism, as well as intact LDL or HDL ...
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