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Isolation and identification of bluetongue virus
Journal of Virological Methods, 2000Bluetongue virus (BTV) is an arthropod-borne orbivirus that infects sheep, wild ruminants and occasionally cattle. Detection and specific identification of BTV is a multistep process. The first step involves the isolation of the virus from the animal's blood or other tissues, followed by inoculation of embryonating chicken eggs (ECE).
Alfonso Clavijo, G C Dulac, A Afshar
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The Typing of Bluetongue Virus
Research in Veterinary Science, 1971SUMMARY Typing of bluetongue virus has been carried out by applying the technique of plaque inhibition. This method has advantages over serum virus neutralization tests in eggs, tubes or petri dishes.
F G, Davies, N K, Blackburn
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Immunobiology of bluetongue virus
Veterinary Immunology and Immunopathology, 1987Following BTV infection or vaccination, sheep develop both anti-virus antibody (which may include neutralizing antibody) and a cellular immune response. Yet, it still is unclear what aspects of the response are most critical in preventing infection and disease from this virus.
K T, Schultz, F B, Grieder
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Morphology of bluetongue virus
Virology, 1969Abstract Electron micrographs of highly purified bluetongue virus, negatively stained with phosphotungstate, are presented. The morphological details suggest that the capsid of the virion consists of a single layer of 32 capsomeres arranged in 5:3:2 symmetry. The size of the virion is about 54 mμ, and it has no envelope.
H J, Els, D W, Verwoerd
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American Journal of Veterinary Research, 1972
SUMMARY Bluetongue (bt) viremia in Saanen goats was assayed by inoculating blood into embryonating chicken eggs. High-titer viremia consistently occurred in Saanen goats inoculated with either BT 8 or 63-66B strain of bt virus. Low-titer viremia consistently occurred in Saanen goats inoculated with 4 other strains of bt virus.
A J, Luedke, E I, Anakwenze
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SUMMARY Bluetongue (bt) viremia in Saanen goats was assayed by inoculating blood into embryonating chicken eggs. High-titer viremia consistently occurred in Saanen goats inoculated with either BT 8 or 63-66B strain of bt virus. Low-titer viremia consistently occurred in Saanen goats inoculated with 4 other strains of bt virus.
A J, Luedke, E I, Anakwenze
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The Evolutionary Dynamics of Bluetongue Virus
Journal of Molecular Evolution, 2010Bluetongue virus (BTV) is a midge-borne member of the genus Orbivirus that causes an eponymous debilitating livestock disease of great agricultural impact and which has expanded into Europe in recent decades. Reassortment among the ten segments comprising the double-stranded (ds) RNA genome of BTV has played an important role in generating the epidemic
Carpi, Giovanna +2 more
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The Replication of Bluetongue Virus
1990Bluetongue virus (BTV) replicates in the cytoplasm of a wide variety of cell types and infection ultimately leads to cell death. The studies of Verwoerd, Huismans and others in the late 1960s and continuing to the present (see Chap. 2, this volume) on the double-stranded, segmented genomic RNA (Verwoerd 1969; Verwoerd et al. 1970), the bishelled nature
B T, Eaton, A D, Hyatt, S M, Brookes
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Nontransmission of bluetongue virus by embryos from bluetongue virus-infected sheep
American Journal of Veterinary Research, 1988SUMMARY Donor sheep were infected either by bites of bluetongue virus (btv)-infected (serotype 11, “Texas Station strain”) Culicoides variipennis or by inoculation with 100,000 median chicken embryo intravascular lethal doses of btv (serotype 11) from a suspension made from infected C variipennis.
W C, Hare +7 more
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Veterinary Clinics of North America: Food Animal Practice, 1994
Bluetongue (BLU) is a noncontagious viral disease. The virus is a member of the Orbivirus genus and serves as the prototype virus of the genus. BLU is primarily a disease of domestic ruminants, some wild ruminants, and, recently, domestic dogs. The disease is caused by 1 of 24 different serotypes of virus that are distributed worldwide.
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Bluetongue (BLU) is a noncontagious viral disease. The virus is a member of the Orbivirus genus and serves as the prototype virus of the genus. BLU is primarily a disease of domestic ruminants, some wild ruminants, and, recently, domestic dogs. The disease is caused by 1 of 24 different serotypes of virus that are distributed worldwide.
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No evidence of bluetongue virus in Switzerland
Veterinary Microbiology, 2006We report the results of the first survey for antibody against bluetongue virus (BTV) that was conducted in Switzerland in the year 2003. In a nationwide cross-sectional study with partial verification, 2437 cattle sera collected from 507 herds were analysed using competitive enzyme-linked immunosorbent assays (c-ELISA). To adjust for misclassification,
A, Cagienard +4 more
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