Results 121 to 130 of about 695,505 (348)
Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
FEBS Open Bio, EarlyView.We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
wiley +1 more source
GAIMS Journal of Medical SciencesBackground Body fluids obtained from sterile body sites are often expected to be devoid of any pathogenic or commensal microorganisms. The cause of infected body fluids could be any pathological agents or skin contaminants that may be harbored in ...
Brihadishwaran Thiyagarajan +3 more
doaj +1 more source
FEBS Open Bio, EarlyView.Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.This study addressed how a senior research thesis is perceived by undergraduate students. It assessed students' perception of research skills, epistemological beliefs, and career goals in Biochemistry (science) and BDC (science‐business) students. Completing a thesis improved confidence in research skills, resilience, scientific identity, closed gender‐
Celeste Suart +4 more
wiley +1 more source
SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Forensic validation of a combined analysis of mRNA and miRNA markers for precise tissue origin inferences of five kinds of body fluids by RT‐qPCR [PDF]
, 2023 Xuebing Chen, Hui Xu, Bofeng Zhu
openalex +1 more source
From Manual Microscopy to Automated Cell Counters for First Line Screening of Body Fluids : “But not without a special body fluid mode” [PDF]
, 2016 Cellular analysis of body fluids, including cerebrospinal fluid and serous fluids, provides essential information in the differential diagnosis. Improper techniques and inaccurate results may lead to the under or over-diagnosis of disorders such as ...
Fleming, C.K.A. (Chérina)
core
FEBS Open Bio, EarlyView.What factors make for an effective digital learning tool in Higher Education? This systematic review identifies elements of a digital tool that published examples reveal to be features of an engaging and impactful digital tool. A systematic literature search yielded 25 research papers for analysis.
Akmal Arzeman +4 more
wiley +1 more source
Rapid evolution of body fluid regulation following independent invasions into freshwater habitats [PDF]
, 2012 Carol Eunmi Lee +2 more
openalex +1 more source