Results 91 to 100 of about 9,351,631 (289)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Changes in the Infrared Thermographic Response of the Triceps Suralis Muscle During Ankle Flexion–Extension Until Exhaustion in Healthy Adults

open access: yesSports
Background: Infrared thermography and acute muscle fatigue are often correlated in sports medicine for assessing muscle health during and after exercise, but there are no known studies describing the response throughout exercise; therefore, this study ...
Alessio Cabizosu   +2 more
doaj   +1 more source

Hypothalamus and Regulation of Body Temperature.

open access: yesExperimental Biology and Medicine, 1935
Recent work1 has shown that the hypothalamus plays an important part in the regulation of body temperature.
Ranson, S. W., Ingram, W. R.
openaire   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Post-Exercise Recovery in Paralympic Athletes: A Narrative Review of Physiological Considerations and Practical Applications

open access: yesApplied Sciences
Paralympic athletes are challenged by unique systemic strain due to impairment-related physiological and psychological stressors. This study aims to synthesize the current evidence regarding post-exercise recovery modalities in Paralympic athletes ...
Exal Garcia-Carrillo   +8 more
doaj   +1 more source

MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation

open access: yesFEBS Open Bio, EarlyView.
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima   +8 more
wiley   +1 more source

Skin wetness perception across body sites in children and adolescents aged 7–16 years old

open access: yesExperimental Physiology
Human skin wetness perception relies on the multisensory integration of thermal and mechanical cues during contact with moisture. Yet, it is unknown whether children and adolescents perceive skin wetness similarly to younger and older adults.
Alessandro Valenza   +11 more
doaj   +1 more source

Evaluating GenAI‐produced feedback on undergraduate bioscience essays against good higher education feedback practice

open access: yesFEBS Open Bio, EarlyView.
This pilot study investigates the potential of Generative AI to provide formative feedback to students. ChatGPT was prompted to provide feedback on Year 1 Bioscience essays, which were evaluated against established good feedback practices. GenAI‐authored feedback had useful elements, but was limited in scope. GenAI may have potential to provide instant,
Annabel Court   +3 more
wiley   +1 more source

GAD65 Antibody ELISA With Extended Reportable Range: Validation and Guidance for Neurological Practice

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To (1) validate GAD65‐ELISA detection and quantification for type 1 diabetes mellitus and autoimmune neurological diagnoses, (2) correlate ELISA results (reference range < 5 IU/mL) with established radioimmunoprecipitation assay (RIA; ≤ 0.02 nmol/L), and (3) define ELISA clinical utility and pitfalls.
Andrew McKeon   +11 more
wiley   +1 more source

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