Results 131 to 140 of about 22,611,724 (309)
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
Multiporphyrin Arrays Assembled Through Hydrogen Bonding
Although relatively weak in isolation, composite H-bonds can be used as an advantage for the assembly of relatively robust and well-defined arrays of molecular components.
Gunter, Maxwell John
core +1 more source
Numerical Modelling of Void Closure Diffusion Model
A void closure analytic model for the diffusion bonding of titanium TC4 alloy is developed in this study, in which an FEA-based deformation mechanism is coupled with a numerical analysis for diffusion. The focus was to evaluate the effect of pressure and
Muhammad Akif +3 more
doaj +1 more source
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
Adenosine triphosphate as a modulator of protein interactions and stability
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
The effect of metalation on antimicrobial piscidins imbedded in normal and oxidized lipid bilayers. [PDF]
Dreab A, Bayse CA.
europepmc +1 more source
Network and Risk Analysis of Surety Bonds
Surety bonds are financial agreements between a contractor (principal) and obligee (project owner) to complete a project. However, most large-scale projects involve multiple contractors, creating a network and introducing the possibility of incomplete obligations to propagate and result in project failures.
Tamara Broderick +5 more
openaire +3 more sources
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley +1 more source

