Results 51 to 60 of about 6,855,519 (278)

Invisible but not inaccessible—Revealing transient oligomers formed by intrinsically disordered proteins with solution NMR and complementary methods

open access: yesFEBS Letters, EarlyView.
Transient oligomers formed by intrinsically disordered proteins may be ‘invisible’ to direct detection yet remain accessible to solution NMR through equilibrium‐exchange measurements and pressure‐jump experiments. Complementary methods report on mass, stoichiometry, selected distance distributions, morphology, and internal packing.
Martin D. Gelenter, Ad Bax
wiley   +1 more source

Direct localized boundary-domain integro-differential formulations for physically nonlinear elasticity of inhomogeneous body [PDF]

open access: yes, 2005
A static mixed boundary value problem (BVP) of physically nonlinear elasticity for a continuously inhomogeneous body is considered. Using the two-operator Green-Betti formula and the fundamental solution of an auxiliary linear operator, a non-standard ...
Mikhailov, SE
core   +4 more sources

On the estimation of the functional

open access: yesLietuvos Matematikos Rinkinys, 2007
There is considered the functional inf(sup|f (i)(t)), The supremum is taken by i = 0, 1, 2, the infimum is taken by the function set KC2([0, 1]), with boundary conditions f (0) = 1,  f'(0) = 1,  f (1) = 0,  f'(1) = 0.
Gintaras Puriuškis
doaj   +1 more source

Comparing the paths of Hedgehog release in artificial and natural settings

open access: yesFEBS Letters, EarlyView.
Hh morphogens require solubilization factors to overcome their tight association with the plasma membrane and signal to distant cells, but the precise functions of these factors are controversial. Here, we use published in vivo observations as the most relevant evidence to test their suggested functions and discuss contradictions and gaps between in ...
Janna Puschmann, Kay Grobe
wiley   +1 more source

Spatial and single‐nuclei transcriptomics reveals idiosyncratic and generic patterns in papillary and anaplastic thyroid cancers

open access: yesMolecular Oncology, EarlyView.
Matched spatial transcriptomics and single‐nuclei RNA‐seq were generated for anaplastic and BRAFV600E papillary thyroid cancers revealing generic and tumor‐specific states occurring in cancer cells and in the tumor microenvironment. In this context, cancer dedifferentiation mirrored organoid maturation through ordered thyroid marker gain/loss ...
Adrien Tourneur   +11 more
wiley   +1 more source

Boundary problem for the singular heat equation

open access: yesKarpatsʹkì Matematičnì Publìkacìï, 2017
The scheme for solving of a mixed problem with general boundary conditions is proposed for a heat equation \[a(x)\frac{\partial T}{\partial \tau}= \frac{\partial}{\partial x} \left(\lambda(x)\frac{\partial T}{\partial x}\right)\] with coefficient $a(x ...
O.V. Makhnei
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Solution of certain problem with nonlocal boundary condition for one-dimensional wave equation

open access: yesВестник Самарского университета: Естественнонаучная серия
In this paper, we study a problem with nonlocal boundary condition for one-dimensional wave equation. To prove the solvability of the problem we construct biortogonal basis consisting of eigen- and adjoint-functions of not self adjoint operator.
Elvira E. Gasanova, Ludmila S. Pulkina
doaj   +1 more source

DECREASING OF MECHANISMS DYNAMIC LOADING AT THE TRANSIENT STATE

open access: yesNauka ta progres transportu, 2015
Purpose. It is necessary to select modes of motion to reduce the dynamic loads in the mechanisms. This choice should be made on optimization basis. The purpose of research is to study methods of synthesis regimes of mechanisms and machines motion that ...
V. S. Loveikin, Yu. O. Romasevich
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

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