Results 31 to 40 of about 161,440 (216)

Avery fixed point theorem applied to Hammerstein integral equations

Electronic Journal of Differential Equations, 2019
We apply a recent Avery et al. fixed point theorem to the Hammerstein integral equation $$ x(t)=\int^{T_2}_{T_1}G(t,s)f(x(s))\,ds, \quad t\in[T_1,T_2].
Paul W. Eloe, Jeffrey T. Neugebauer
doaj  

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Fractional-order boundary value problem with Sturm-Liouville boundary conditions

Electronic Journal of Differential Equations, 2015
Using the new conformable fractional derivative, which differs from the Riemann-Liouville and Caputo fractional derivatives, we reformulate the second-order conjugate boundary value problem in this new setting.
Douglas R. Anderson, Richard I. Avery
doaj  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Boundary value problems for hypergenic function vectors

Journal of Inequalities and Applications, 2018
This article mainly studies the boundary value problems for hypergenic function vectors in Clifford analysis. Firstly, some properties of hypergenic quasi-Cauchy type integrals are discussed. Then, by the Schauder fixed point theorem the existence of the
Guiling Zhang, Chong Li, Yonghong Xie
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Boundary value problem with fractional p-Laplacian operator

Advances in Nonlinear Analysis, 2016
The aim of this paper is to obtain the existence of solution for the fractional p-Laplacian Dirichlet problem with mixed derivatives tDTα(|0Dtαu(t)|p-20Dtαu(t)) = f(t,u(t)), t ∈ [0,T], u(0) = u(T) = 0, where 1/p < α < 1, 1 < p < ∞ and f : [0,T] × ℝ → ℝ ...
Torres Ledesma César
doaj   +1 more source

The crystal structure of the Borrelia burgdorferi nicotinamidase BBE22 resolves a long‐standing annotation error

FEBS Open Bio, EarlyView.
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source