Results 111 to 120 of about 75,209 (310)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Lower bounds for the Trotter error

Physical Review A
18 pages, 3 ...
Alexander Hahn, Paul Hartung, Daniel Burgarth, Paolo Facchi, Kazuya Yuasa   +4 more
openaire   +2 more sources

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Bounded Error Data Compression at Constant Rate

, 1978
International Telemetering Conference Proceedings / November 14-16, 1978 / Hyatt House Hotel, Los Angeles, CaliforniaWe present a technique to compress M-bit data to N-bit data with a bounded error between the original and de-compressed data. The bounded
Shea, Alicia, Evans, Norman E., Gonsalves, Robert A.   +2 more
core  

Enumerating Error Bounded Polytime Algorithms Through Arithmetical Theories [PDF]

We consider a minimal extension of the language of arithmetic, such that the bounded formulas provably total in a suitably-defined theory à la Buss (expressed in this new language) precisely capture polytime random functions.
Melissa Antonelli, Davoli, Davide, Pistone, Paolo, Ugo Dal Lago, Isabel Oitavem, Dal Lago, Ugo, Davide Davoli, Antonelli, Melissa, Oitavem, Isabel, Paolo Pistone   +9 more
core   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Enumerating Error Bounded Polytime Algorithms Through Arithmetical Theories

, 2023
We consider a minimal extension of the language of arithmetic, such that the bounded formulas provably total in a suitably-defined theory \`a la Buss (expressed in this new language) precisely capture polytime random functions.
Lago, Ugo Dal, Davoli, Davide, Pistone, Paolo, Antonelli, Melissa, Oitavem, Isabel   +4 more
core  

Constants within Error Estimates for Legendre-Galerkin Spectral Approximations of Control-Constrained Optimal Control Problems

Abstract and Applied Analysis, 2014
Explicit formulae of constants within the a posteriori error estimate for optimal control problems are investigated with Legendre-Galerkin spectral methods. The constrained set is put on the control variable.
Jianwei Zhou
doaj   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source