Results 31 to 40 of about 88,982 (306)
FEBS Open Bio, EarlyView.CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
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FEBS Open Bio, EarlyView.Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
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Advances in Civil Engineering, 2021 For calculating the thin-walled closed curved box girder caused by the temperature gradient of the internal force and displacement, based on the fundamental differential equation of the curve beam and the principle of minimum energy, set a reverse ...
Zeying Yang +5 more
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FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
BuildingsBox beams constructed earlier were prone to inadequate bending capacity owing to low construction standards, overloading, and environmental degradation.
Shengwei Nong +8 more
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FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
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Nihon Kikai Gakkai ronbunshu, 2019 In this paper, the equations of in-plane and out-of-plane displacements, in-plane stress distributions, and Mises stress of the rectangular plate after buckling are composed using the well-known buckling deflection shape, based on the plane stress state ...
Katsuya FURUSU +4 more
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Advanced Engineering Materials, EarlyView.This study demonstrates an efficient recycling route for out‐of‐spec AlSi10Mg atomized powders through compaction and arc remelting followed by suction casting. By correlating compaction load, cooling rate, and resulting microstructure, we show that intermediate pressures (50–80 kN) and rapid cooling refine dendrites, reduce porosity, and enhance ...
Mila Christy de Oliveira +4 more
wiley +1 more source
Flexural–torsional buckling of thin-walled composite box beams
, 2007 Buckling of an axially loaded thin-walled laminated composite is studied. A general analytical model applicable to the flexural, torsional and flexural–torsional buckling of a thin-walled composite box beam subjected to axial load is developed.
Lee, Jaehong, Vo, Thuc
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Advanced Engineering Materials, EarlyView.It is shown that laser ablation pretreatment under oxygen‐free conditions enables copper–aluminium bonding at significantly lower deformation degrees and improved properties compared to mechanical brushing. Laser ablation further increases interface contact area and induces favourable residual stress states and microstructural compatibility ...
Khemais Barienti +11 more
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