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Electroporation of Bradyrhizobium japonicum

Molecular and General Genetics MGG, 1990
Electroporation offers a fast, efficient and reproducible way to introduce DNA into bacteria. We have successfully used this technique to transform two commercially important strains of Bradyrhizobium japonicum, the nitrogen-fixing soybean symbiont. Initially, electroporation conditions were optimized using plasmid DNA which had been prepared from the ...
Mary Lou Guerinot   +2 more
openaire   +1 more source

Viability of Bradyrhizobium japonicum bacteriods

Archives of Microbiology, 1987
Homogenates from soybean nodules, formed by 12 strains of Bradyrhizobium japonicum, were plated into yeast-extract mannitol agar containing 3 or 37 g mannitol 1-1. Viable counts ranged from 8.298 to 11.265 log10 cells-gram nodule-1. When monitored over the life cycle of the symbiosis, the viability of strains USDA 110 and USDA 123 increased with days ...
T. R. McDermott   +2 more
openaire   +1 more source

Degradation of catechin by Bradyrhizobium japonicum

Biodegradation, 1997
Rhizobia utilize phenolic substances as sole carbonsource. Bradyrhizobium japonicum utilizescatechin, a unit of condensed tannin as carbonsource. To establish the degradative pathway ofcatechin, the products of catechin degradation wereisolated by paper chromatography and TLC andidentified by HPLC, UV, IR and NMR spectra.
Waheeta Hopper, A. Mahadevan
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???????????????? ???????????????????? ?????????????? ?????? ???? ???????????? ?? ?????????????????? ???????????????? ???????????????????????????? Bradyrhizobium japonicum

2011
???????????????? ???????????????????? ???????????????? ?????????????????? ???????????????????????? ???????????????? ?????? ?? ???????????? ?????????????????? ???? ???????????????????? ???????????????????? ?????????????? ?????? ???????????? ????????????. ?????????????? ?????? ???? ???????????????? ???? ???????????????? ???? ?? ???????????????? ??????????
  +6 more sources

BRADYRHIZOBIUM JAPONICUM AND PHOSPHOBACTERIA FOR SOYBEAN

Madras Agricultural Journal, 1998
The effect of individual and combined inoculations of Bradyrhizobiumand phosphobacteria along with application of super phosphate. rock phosphate and FYM on the growth and yield of soybean were studied. The combined inoculation of Bradyrhizobium and phosphobacteria was proved to be better than individual inoculations. The combined inoculations with FYM+
SHEERIN S   +2 more
openaire   +1 more source

A Motility Revertant of the ndvB Mutant of Bradyrhizobium japonicum

Current Microbiology, 2003
A motility revertant of a Bradyrhizobium japonicum ndvB mutant was isolated and characterized. The ndvB mutants of B. japonicum have been reported to be osmotically sensitive, as well as defective in motility, periplasmic cyclic beta-(1-->3), (1-->6)-D-glucan synthesis, and symbiosis with soybean.
Rongji, Chen   +2 more
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Analysis of the secretome of the soybean symbiont Bradyrhizobium japonicum

Journal of Biotechnology, 2009
Proteins from the supernatant of Bradyrhizobium japonicum were separated by two-dimensional gel electrophoresis and stained with Coomassie. This revealed more than 100 protein spots. Sixty-eight proteins were identified by mass spectrometry. Thirty-five are predicted to contain an N-terminal signal peptide characteristic for proteins transported by the
Hempel, Jana   +3 more
openaire   +3 more sources

Regulation of nod gene expression in Bradyrhizobium japonicum

Molecular and General Genetics MGG, 1988
The best inducers of nod::lacZ translational fusions in Bradyrhizobium japonicum are isoflavones, primarily genistein and daidzein. Upstream of the nodABC genes in B. japonicum is a novel gene, nodY, which is coregulated with nodABC. Measurements of the activity of lacZ fusions to the nodD gene of B.
Z, Banfalvi   +4 more
openaire   +2 more sources

Genes for a second terminal oxidase in Bradyrhizobium japonicum

Archives of Microbiology, 1992
Bradyrhizobium japonicum possesses a mitochondria-like respiratory chain terminating with an aa3-type cytochrome c oxidase. The gene for subunit I of this enzyme (coxA) had been identified and cloned previously via heterologous hybridization using a Paracoccus denitrificans DNA probe. In the course of these studies, another B.
Bott M, Preisig O, Hennecke H
openaire   +3 more sources

???????????????? ?????????????????????????????? ?? ???????????????????????????????? ?????????????????????? ???? ???????????????????????????? ??????, ?????????????????????????? Tn5-?????????????????? Bradyrhizobium japonicum

2009
Influence of the inoculation of soybean by new Tn5-mutants of Bradyrhizobium japonicum 646 on seed productivity and change of nitrogen fixing activity (NFA) and photosynthetic rate (PR) on the different stages of plant development were studied in vegetative experiment.
openaire   +2 more sources

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