Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Analisis Break Even Point USAha Peternakan Ayam Broiler Di Kecamatan Limbangan Kabupaten Kendal Analysis of Break Even Point at Broiler Farm in Limbangan Distric Kendal Regency [PDF]
The study aims to determine the value ( Break Even Point ) BEP units, sales value and the difference value BEP units and sales in stratum I, II and III on broiler chicken farm in the district partnership Limbangan Kendal. This study was conducted in June-
Handayani, M. (Migie) +2 more
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ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui +10 more
wiley +1 more source
Cutaneous Melanoma Drives Metabolic Changes in the Aged Bone Marrow Immune Microenvironment
Melanoma, the deadliest form of skin cancer, increasingly affects older adults. Our study reveals that melanoma induces changes in iron and lipid levels in the bone marrow, impacting immune cell populations and increasing susceptibility to ferroptosis.
Alexis E. Carey +12 more
wiley +1 more source
Lessons Learned From a Delayed‐Start Trial of Modafinil for Freezing of Gait in Parkinson's Disease
ABSTRACT Objective Freezing of gait (FOG) in people with Parkinson's disease (PwPD) is debilitating and has limited treatments. Modafinil modulates beta/gamma band activity in the pedunculopontine nucleus (PPN), like PPN deep brain stimulation. We therefore tested the hypothesis that Modafinil would improve FOG in PwPD.
Tuhin Virmani +8 more
wiley +1 more source
Analisis Titik Impas USAhatani Tanaman Ketepeng Cina (Cassia Alata L) pada PT. Srikaya Sega Utama Banjarbaru [PDF]
The study aims to determine the technical processing of candle bush total cost, total revenue, income and break even point. The method used in this research is survey method with observation technique participation.
Mahdalena, Z. (Zulipah) +1 more
core +1 more source
ABSTRACT Objective People with epilepsy (PWE) may experience cognitive deficits but fail to undergo formal evaluation. This study compares cognitive status between PWE and healthy controls in the West African Republic of Guinea. Methods A cross‐sectional, case–control study was conducted in sequential recruitment phases (July 2024–July 2025) at Ignace ...
Maya L. Mastick +14 more
wiley +1 more source
Titik Impas dan Bauran Pemasaran Bunga Rosella pada UD Bali Gendis di Desa Dawan Kelod Kecamatan Dawan Kabupaten Klungkung [PDF]
Break Even and Marketing Mix of Rosella Flowers at UD Bali Gendis in The Village Dawan Kelod, District of Dawan, Sub District of Klungkung The research was conducted from early November 2012 until the end of January 2013 with the aim to determine the ...
ARILA, J. (JESSICA) +2 more
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Applying an Ethical Lens to the Treatment of People With Multiple Sclerosis
ABSTRACT The practice of neurology requires an understanding of clinical ethics for decision‐making. In multiple sclerosis (MS) care, there are a wide range of ethical considerations that may arise. These involve shared decision‐making around selection of a disease‐modifying therapy (DMT), risks and benefits of well‐studied medications in comparison to
Methma Udawatta, Farrah J. Mateen
wiley +1 more source
Methodological aspects of evaluation and areas break-even analysis [PDF]
Описано результати дослідження теорії CVP-аналізу. Розглянуто базові положення цієї теорії та визначено основні методологічні недоліки. Запропоновано новий методичний підхід щодо визначення зони беззбитковості, яка забезпечує більш високу достовірність ...
Chymshyt, Serhiy +2 more
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