Results 151 to 160 of about 282,442 (237)

Development of an HEK293 Suspension Cell Culture Medium, Transient Transfection Optimization Workflow, and Analytics for Batch rAAV Manufacturing

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Recombinant adeno associated virus (rAAV) vectors have become popular delivery vehicles for in vivo gene therapies, but demand for rAAVs continues to outpace supply. Platform processes for rAAV production are being developed by many manufacturers, and transient chemical transfection of human embryonic kidney 293 (HEK293) cells is currently the
Erica A. Green, Qiang Fu, Nelson Ndahiro, Thomas M. Leibiger, Yongdan Wang, Yongsuk Lee, Kelvin H. Lee, Michael Betenbaugh, Seongkyu Yoon, David J. McNally   +9 more
wiley   +1 more source

Flux Sampling Suggests Metabolic Signatures of High Antibody‐Producing CHO Cells

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Chinese hamster ovary (CHO) cells remain the industry standard for producing numerous therapeutic proteins, particularly monoclonal antibodies (mAbs). However, achieving higher recombinant protein titers remains an ongoing challenge and a fundamental understanding of the cellular mechanism driving improved bioprocess performance remains ...
Kate E. Meeson, Joanne Watson, Susan Rosser, Ellie Hawke, Andrew Pitt, Tessa Moses, Leon Pybus, Magnus Rattray, Alan J. Dickson, Jean‐Marc Schwartz   +9 more
wiley   +1 more source

Machine learning analysis for the association between breast feeding and metabolic syndrome in women. [PDF]

Sci Rep
Lee JS, Choi ES, Lee H, Son S, Lee KS, Ahn KH.   +5 more
europepmc   +1 more source

Determinants of early initiation of breast feeding during COVID-19 pandemic among urban-dwelling mothers from Tigray, Northern Ethiopia: a community-based cross-sectional study. [PDF]

BMJ Open, 2023
Gebretsadik GG, Berhe K, Gebregziabher H.   +2 more
europepmc   +1 more source

Physiology and Robustness of Yeasts Exposed to Dynamic pH and Glucose Environments

Biotechnology and Bioengineering, EarlyView.
The authors investigated how three Saccharomyces cerevisiae strains respond to dynamic substrate and pH oscillations using dynamic microfluidic single‐cell cultivation (dMSCC) and intracellular biosensors. By combining performance and robustness analysis of selected intracellular parameters, growth and morphological analysis, the authors identified ...
Luca Torello Pianale, Luisa Blöbaum, Alexander Grünberger, Lisbeth Olsson   +3 more
wiley   +1 more source

Thoughts on Breast-Feeding [PDF]

, 1965
Schneidau, Calista R.
core   +1 more source

Systematic Investigation of Impact of Antifoam and Extracellular Vesicles on Fouling of Hollow Fiber Filters in Intensified Perfusion Processes Highlights the Key Impact of Antifoam

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Ultra‐high cell density perfusion cultures are becoming increasingly attractive for biologics manufacturing due to their ability to boost productivity and offer a flexible manufacturing footprint. However, these intensified perfusion processes pose challenges, particularly concerning the performance of hollow fiber filters used as cell ...
Sri Ranganayaki Madabhushi, Qianying Zuo, Carol Zhou, Chelsea Phuangthong, Nathaniel Ruppert, Tiffany Tang, Francisco Pelaez   +6 more
wiley   +1 more source

Temporal Galactose‐Manganese Feeding in Fed‐Batch and Perfusion Bioreactors Modulates UDP‐Galactose Pools for Enhanced mAb Glycosylation Homogeneity

Biotechnology and Bioengineering, EarlyView.
This study investigates the temporal effects of galactose and manganese on the N‐glycosylation of Trastuzumab (mAb) antibody during fed‐batch and perfusion bioprocessing modes to reveal the influence of feeding schedules on mAb N‐glycosylation patterns. These findings will aid in building process control schemes for mAb bioprocessing.
Aron Gyorgypal, Erica Fratz‐Berilla, Casey Kohnhorst, David N. Powers, Shishir P. S. Chundawat   +4 more
wiley   +1 more source

Unravelling taboos and cultural beliefs associated with hidden hunger among pregnant and breast-feeding women in Buyende district Eastern Uganda. [PDF]

J Ethnobiol Ethnomed
Tugume P, Mustafa AS, Walusansa A, Ojelel S, Nyachwo EB, Muhumuza E, Nampeera M, Kabbale F, Ssenku JE.   +8 more
europepmc   +1 more source

Balancing Doses of EL222 and Light Improves Optogenetic Induction of Protein Production in Komagataella phaffii

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Komagataella phaffii, also known as Pichia pastoris, is a powerful host for recombinant protein production, in part due to its exceptionally strong and tightly controlled PAOX1 promoter. Most K. phaffii bioprocesses for recombinant protein production rely on PAOX1 to achieve dynamic control in two‐phase processes.
Shannon M. Hoffman, Sebastián Espinel‐Ríos, Sarah K. Kwartler, Makoto A. Lalwani, José L. Avalos   +4 more
wiley   +1 more source