Results 81 to 90 of about 240,816 (302)

haesleinhuepf/napari-brightness-contrast: 0.1.7

open access: yes, 2022
Bug fixes The plugin crashed when a shapes layer was added (thanks to @psobolewskiPhD for reporting and fixing!) Simplified testing with pytest (thanks to @psobolewskiPhD
Robert Haase, Peter Sobolewski
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Brightness transformation (higher brightness value) using leuven image.

open access: yes, 2019
Brightness transformation (higher brightness value) using leuven image.
Siok Yee Tan (6172052)   +2 more
core   +1 more source

Volume Bragg grating external cavities for the passive phase locking of high-brightness diode laser arrays: theoretical and experimental study

open access: yes, 2011
International audienceWe describe the theoretical modeling of the external-cavity operation of a phase-locked array of diode lasers in two configurations, the self-imaging cavity based on the Talbot effect and the angular-filtering cavity.
Larkins, Eric   +8 more
core   +2 more sources

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

open access: yesFEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour   +2 more
wiley   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

open access: yesFEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová   +2 more
wiley   +1 more source

The bright side of boredom [PDF]

open access: yesFrontiers in Psychology, 2014
Boredom proneness is commonly assessed and measured using self-report scales and questionnaires. The only full-scale measure of boredom that has been extensively used to assess boredom proneness is the Boredom Proneness Scale (BPS) (Farmer and Sundberg, 1986).
openaire   +3 more sources

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

Brightness transformation (lower brightness value) using leuven image.

open access: yes, 2019
Brightness transformation (lower brightness value) using leuven image.
Siok Yee Tan (6172052)   +2 more
core   +1 more source

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